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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharide

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Author(s):
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Cabral, Lucelia [1] ; Persinoti, Gabriela F. [1] ; Paixao, Douglas A. A. [1] ; Martins, Marcele P. [2, 1] ; Morais, Mariana A. B. [1] ; Chinaglia, Mariana [2, 1] ; Domingues, Mariane N. [1] ; Sforca, Mauricio L. [3] ; Pirolla, Renan A. S. [1] ; Generoso, Wesley C. [1] ; Santos, Clelton A. [1] ; Maciel, Lucas F. [1] ; Terrapon, Nicolas [4, 5] ; Lombard, Vincent [4, 5] ; Henrissat, Bernard [6, 7] ; Murakami, Mario T. [1]
Total Authors: 16
Affiliation:
[1] Brazilian Ctr Res Energy & Mat, Brazilian Biorenewables Natl Lab, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Grad Program Funct & Mol Biol, Campinas, SP - Brazil
[3] Brazilian Ctr Res Energy & Mat, Brazilian Biosci Natl Lab, Campinas, SP - Brazil
[4] INRA, USC 1408, AFMB, F-13288 Marseille - France
[5] Aix Marseille Univ, CNRS, Architecture & Fonct Macromol Biol, Marseille - France
[6] Tech Univ Denmark, Dept Biotechnol & Biomed DTU Bioengn, DK-2800 Lyngby - Denmark
[7] King Abdulaziz Univ, Dept Biol Sci, Jeddah - Saudi Arabia
Total Affiliations: 7
Document type: Journal article
Source: NATURE COMMUNICATIONS; v. 13, n. 1 FEB 2 2022.
Web of Science Citations: 0
Abstract

Here, Cabral et al., perform a multi-omics analysis of the gut microbiome of capybara, the largest living rodent, unveiling enzymatic mechanisms for the breakdown of lignocellulosic biomass, and report two undescribed families of carbohydrate-active enzymes. The largest living rodent, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, we elucidate the microbial community composition, enzymatic systems and metabolic pathways involved in the conversion of dietary fibers into short-chain fatty acids, a main energy source for the host. In this microbiota, the unconventional enzymatic machinery from Fibrobacteres seems to drive cellulose degradation, whereas a diverse set of carbohydrate-active enzymes from Bacteroidetes, organized in polysaccharide utilization loci, are accounted to tackle complex hemicelluloses typically found in gramineous and aquatic plants. Exploring the genetic potential of this community, we discover a glycoside hydrolase family of beta-galactosidases (named as GH173), and a carbohydrate-binding module family (named as CBM89) involved in xylan binding that establishes an unprecedented three-dimensional fold among associated modules to carbohydrate-active enzymes. Together, these results demonstrate how the capybara gut microbiota orchestrates the depolymerization and utilization of plant fibers, representing an untapped reservoir of enzymatic mechanisms to overcome the lignocellulose recalcitrance, a central challenge toward a sustainable and bio-based economy. (AU)

FAPESP's process: 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications
Grantee:Mário Tyago Murakami
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/19995-0 - Analysis of structural and functional diversity of GH43 enzymes from Xanthomonas axonopodis pv. citri: biological implications and potential biotechnological applications
Grantee:Mariana Abrahão Bueno de Morais
Support type: Scholarships in Brazil - Post-Doctorate