Cytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cells

Author(s)

Marlus da Silva Pedrosa ^[1] ; Tomaz Alves ^[2] ; Juliana Garuba Rahhal ^[3] ; Fernando Neves Nogueira ^[4] ; Carla Renata Sipert ^[5]

Affiliation

^[1] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil

^[2] University of São Paulo. School of Dentistry. Department of Stomatology - Brasil

^[3] University of São Paulo. School of Dentistry. Department of Restorative Dentistry - Brasil

^[4] University of São Paulo. School of Dentistry. Department of Biomaterials and Oral Biology - Brasil

^[5] University of São Paulo. School of Dentistry. Department of Restorative Dentistry - Brasil

• Document type: Journal article
• Source: Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 22, 2022-03-07.

Abstract

ABSTRACT Objective To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods The culture of hPDLSCs was established. Cell density was set at 2 × 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey’s test (α=0.05). Results At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs. (AU)

• FAPESP's process: 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study
• Grantee: Carla Renata Sipert
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/23158-0 - EFFECT OF PROTEASE ACTIVATED RECEPTOR 1 (PAR1) ACTIVATION ON THE OSTEOGENIC ACTIVITY OF MEMBRANES OF HUMAN PERIODONTAL LIGAMENT MESENCHYMAL STEM CELLS
• Grantee: Marinella Holzhausen Caldeira
• Support Opportunities: Regular Research Grants