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Anti-aquaporin-4 immunoglobulin G colorimetric detection by silver nanoparticles

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Higa, Akemi M. M. ; Moraes, Ariana S. S. ; Shimizu, Flavio M. G. ; Bueno, Raquel G. A. ; Peroni, Luis A. T. ; Strixino, Francisco T. ; Sousa, Nise A. C. G. ; Deffune, Elenice L. ; Bovolato, Ana Livia C. ; Oliveira Jr, Osvaldo N. ; Brum, Doralina G. ; Leite, Fabio L.
Total Authors: 12
Document type: Journal article
Source: Nanomedicine-Nanotechnology Biology and Medicine; v. 41, p. 9-pg., 2022-04-01.

Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory and autoimmune disease whose biomarker is the anti-AQP4-IgG autoantibody that binds to aquaporin-4 (AQP4) protein. We introduced a nanosensor with a sensitivity of 84.6%, higher than the CBA's 76.5%. Using silver nanoparticles (AgNPs), we detected not only seropositive but also some false-negative patients previously classified with CBA. It consisted of AgNPs coated with one of a panel of 5 AQP4 epitopes. The ability in detecting the anti-AQP4-IgG in NMOSD patients depended on the epitope and synergy could be obtained by combining different epitopes. We demonstrated that NMOSD patients could easily be distinguished from healthy subjects and patients with multiple sclerosis. Using the most sensitive AQP461-70 peptide, we established a calibration curve to estimate the concentration of anti-AQP4-IgG in seropositive NMOSD patients. The ability to enhance the accuracy of the diagnosis may improve the prognosis of 10-27% of anti-AQP4-IgG seronegative patients worldwide. (C) 2022 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 15/05283-6 - Nanoimmunosensors development to neuromyelitis optica survey and detection
Grantee:Ariana de Souza Moraes
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
Grantee:Roberto Mendonça Faria
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/06847-0 - Synthesis and characterization of silver nanoparticles applied to immunoglobulins G detection
Grantee:Akemi Martins Higa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants