Anti-aquaporin-4 immunoglobulin G colorimetric detection by silver nanoparticles

Author(s):Show less -

Higa, Akemi M. M. ; Moraes, Ariana S. S. ; Shimizu, Flavio M. G. ; Bueno, Raquel G. A. ; Peroni, Luis A. T. ; Strixino, Francisco T. ; Sousa, Nise A. C. G. ; Deffune, Elenice L. ; Bovolato, Ana Livia C. ; Oliveira Jr, Osvaldo N. ; Brum, Doralina G. ; Leite, Fabio L.

• Document type: Journal article
• Source: Nanomedicine-Nanotechnology Biology and Medicine; v. 41, p. 9-pg., 2022-04-01.

Abstract

Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory and autoimmune disease whose biomarker is the anti-AQP4-IgG autoantibody that binds to aquaporin-4 (AQP4) protein. We introduced a nanosensor with a sensitivity of 84.6%, higher than the CBA's 76.5%. Using silver nanoparticles (AgNPs), we detected not only seropositive but also some false-negative patients previously classified with CBA. It consisted of AgNPs coated with one of a panel of 5 AQP4 epitopes. The ability in detecting the anti-AQP4-IgG in NMOSD patients depended on the epitope and synergy could be obtained by combining different epitopes. We demonstrated that NMOSD patients could easily be distinguished from healthy subjects and patients with multiple sclerosis. Using the most sensitive AQP461-70 peptide, we established a calibration curve to estimate the concentration of anti-AQP4-IgG in seropositive NMOSD patients. The ability to enhance the accuracy of the diagnosis may improve the prognosis of 10-27% of anti-AQP4-IgG seronegative patients worldwide. (C) 2022 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 15/05283-6 - Nanoimmunosensors development to neuromyelitis optica survey and detection
• Grantee: Ariana de Souza Moraes dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
• Grantee: Roberto Mendonça Faria
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/06847-0 - Synthesis and characterization of silver nanoparticles applied to immunoglobulins G detection
• Grantee: Akemi Martins Higa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants