| Full text | |
| Author(s): |
de Souza Oliveira, Patricia F.
;
Faria, Alessandra V. S.
;
Clerici, Stefano P.
;
Akagi, Erica M.
;
Carvalho, Hernandes F.
;
Justo, Giselle Z.
;
Duran, Nelson
;
Ferreira-Halder, Carmen, V
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Journal of Cellular Biochemistry; v. N/A, p. 12-pg., 2022-06-06. |
| Abstract | |
Violacein is a secondary metabolite produced by several microorganisms including Chromobacterium violaceum, and it is already used in food and cosmetics. However, due to its potent anticancer and low side effects, its molecular action needs to be deeply scrutinized. Therefore, the main objective of this study was to evaluate the violacein's ability to interfere with three cancer hallmarks: growth factors receptor-dependent signaling, proliferation, and epithelial-mesenchymal transition (EMT). Violacein has been associated with the induction of apoptosis in colorectal cancer (CRC) cells. Here, we demonstrate that this molecule is also active in CRC spheroids and inhibits cell migration. Violacein treatment reduced the amount of EGFR and AXL receptors in the HT29 cell line. Accordingly, the inhibition of the AKT, ERK, and PKC delta kinases, which are downstream mediators of the signaling pathways triggered by EGFR and AXL, is detected. Another interesting finding was that even when the cells were stimulated with transforming growth factor-beta, the EMT marker (N-cadherin) decreased. Therefore, this study provides further evidence that reinforces the potential of violacein as an antitumor agent, once this biomolecule can "switch off" properties associated with cancer plasticity. (AU) | |
| FAPESP's process: | 17/16625-0 - Importance of the low molecular weight protein tyrosine phosphatase in the tumoral metabolism reprogramming |
| Grantee: | Erica Mie Akagi da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/03593-6 - Tumor-educated platelets and metastasis: relevance of LMWPTP and extracellular vesicles in Colorectal Cancer |
| Grantee: | Stefano Piatto Clerici |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/50938-8 - INCT 2014: in Photonics Applied to Cell Biology |
| Grantee: | Hernandes Faustino de Carvalho |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 20/04409-4 - Gastric cancer spheroids and organoids: protein kinases and extracellular matrix architecture tracking and therapeutic impact |
| Grantee: | Carmen Veríssima Ferreira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/08119-8 - Hematogenous tumor metastasis in colon rectal cancer cells: influence of LMWPTP and 3-bromopyruvate |
| Grantee: | Alessandra Valéria de Sousa Faria |
| Support Opportunities: | Scholarships in Brazil - Doctorate |