Lippia grata essential oil complexed with beta-cyclodextrin ameliorates biochemical and behavioral deficits in an animal model of progressive parkinsonism

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Beserra-Filho, Jose Ivo A. ; Maria-Macedo, Amanda ; Silva-Martins, Suellen ; Custodio-Silva, Ana Claudia ; Soares-Silva, Beatriz ; Silva, Sara Pereira ; Lambertucci, Rafael Herling ; de Souza Araujo, Adriano Antunes ; Lucchese, Angelica Maria ; Quintans-Junior, Lucindo J. ; Santos, Jose Ronaldo ; Silva, Regina H. ; Ribeiro, Alessandra M.

• Document type: Journal article
• Source: METABOLIC BRAIN DISEASE; v. N/A, p. 17-pg., 2022-07-02.

Abstract

Parkinson's disease (PD) is identified by the loss of dopaminergic neurons in the Substantia Nigra pars compacta (SNpc), and is correlated to aggregates of proteins such as alpha-synuclein, Lewy's bodies. Although the PD etiology remains poorly understood, evidence suggests a main role of oxidative stress on this process. Lippia grata Schauer, known as "alecrim-do-mato", "alecrim-de-vaqueiro", "alecrim-da-chapada", is a native bush from tropical areas mainly distributed throughout the Central and South America. This plant species is commonly used in traditional medicine for relief of pain and inflammation conditions, and that has proven antioxidant effects. We evaluated the effects of essential oil of the L. grata after its complexed with beta-cyclodextrin (LIP) on PD animal model induced by reserpine (RES). Behavioral assessments were performed across the treatment. Upon completion the treatment, the animals were euthanized, afterwards their brains were isolated and processed for immunohistochemical and oxidative stress analysis. The LIP treatment delayed the onset of the behavior of catalepsy, decreased the number of oral movements and prevented the memory impairment on the novel object recognition task. In addition, the treatment with LIP protected against dopaminergic depletion in the SNpc and dorsal striatum (STRd), and decreased the alpha-syn immunoreactivity in the SNpc and hippocampus (HIP). Moreover, there was reduction of the oxidative stability index. These findings demonstrated that the LIP treatment has neuroprotective effect in a progressive parkinsonism model, suggesting that LIP could be an important source for novel treatment approaches in PD. (AU)

• FAPESP's process: 18/26609-5 - Effects of myrtenol monoterpene complexed with Beta-cyclodextrin in a mice progressive model of Parkinson's Disease
• Grantee: Suellen Silva Martins
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/20785-8 - Bioprospecting of natural products with potential neuroprotective activity in animal models of Epilepsy and Parkinson's disease.
• Grantee: Alessandra Mussi Ribeiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/03354-3 - PATHOPHYSIOLOGICAL MECHANISMS AND NEUROPROTECTIVE INTERVENTIONS IN A PROGRESSIVE ANIMAL MODEL OF PARKINSON'S DISEASE
• Grantee: Regina Helena da Silva
• Support Opportunities: Regular Research Grants