| Full text | |
| Author(s): Show less - |
Reis, Natany G.
;
Assis, Ana P.
;
Lautherbach, Natalia
;
Goncalves, Dawit A.
;
Silveira, Wilian A.
;
Morgan, Henrique J. N.
;
Valentim, Rafael R.
;
Almeida, Lucas F.
;
Heck, Lilian C.
;
Zanon, Neusa M.
;
Koike, Tatiana E.
;
Santos, Audrei R.
;
Miyabara, Elen H.
;
Kettelhut, Isis C.
;
Navegantes, Luiz C.
Total Authors: 15
|
| Document type: | Journal article |
| Source: | JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE; v. N/A, p. 13-pg., 2022-05-18. |
| Abstract | |
Background Fetal stage is a critical developmental window for the skeletal muscle, but little information is available about the impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in the adult life of male and female animals. Methods Female rats (Wistar Hannover) were fed either a control (1000 IU Vit. D3/kg) or a VDD diet (0 IU Vit. D3/kg) for 6 weeks and during gestation and lactation. At weaning, male and female offspring were randomly separated and received a standard diet up to 180 days old. Results Vitamin D deficiency induced muscle atrophy in the male (M-VDD) offspring at the end of weaning, an effect that was reverted along the time. Following 180 days, fast-twitch skeletal muscles [extensor digitorum longus (EDL)] from the M-VDD showed a decrease (20%; P < 0.05) in the number of total fibres but an increase in the cross-sectional area of IIB (17%; P < 0.05), IIA (19%; P < 0.05) and IIAX (21%; P < 0.05) fibres. The fibre hypertrophy was associated with the higher protein levels of MyoD (73%; P < 0.05) and myogenin (55% %; P < 0.05) and in the number of satellite cells (128.8 +/- 14 vs. 91 +/- 7.6 nuclei Pax7 + in the M-CTRL; P < 0.05). M-VDD increased time to fatigue during ex vivo contractions of EDL muscles and showed an increase in the phosphorylation levels of IGF-1/insulin receptor and their downstream targets related to anabolic processes and myogenic activation, including Ser (473)Akt and Ser (21/9)GSK-3 beta. In such muscles, maternal VDD induced a compensatory increase in the content of calcitriol (two-fold; P < 0.05) and CYP27B1 (58%; P < 0.05), a metabolizing enzyme that converts calcidiol to calcitriol. Interestingly, most morphological and biochemical changes found in EDL were not observed in slow-twitch skeletal muscles (soleus) from the M-VDD group as well as in both EDL and soleus muscles from the female offspring. Conclusions These data show that maternal VDD selectively affects the development of type-II muscle fibres in male offspring rats but not in female offspring rats and suggest that the enhancement of their size and fatigue resistance in fast-twitch skeletal muscle (EDL) is probably due to a compensatory increase in the muscle content of Vit. D in the adult age. (AU) | |
| FAPESP's process: | 19/06517-1 - The effect of maternal vitamin D deficiency on offspring development and muscle protein metabolism |
| Grantee: | Natany Garcia Reis |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/10089-2 - Neural, hormonal and nutritional control of autophagy |
| Grantee: | Isis Do Carmo Kettelhut |
| Support Opportunities: | Research Projects - Thematic Grants |