Photobiomodulation treatments drive osteogenic versus adipocytic fate of bone marrow mesenchymal stem cells reversing the effects of hyperglycemia in diabetes

Author(s)

Bueno, Natalia Pieretti ; Kfouri, Cecilia Cardoso ; Copete, Isabella Nunes ; de Oliveira, Fabiola Singaretti ; Arany, Praveen ; Marques, Marcia Martins ; Ferraz, Emanuela Prado

• Document type: Journal article
• Source: Lasers in Medical Science; v. N/A, p. 10-pg., 2022-04-02.

Abstract

Diabetes mellitus (DM) is a chronic metabolic disease that affects bone metabolism, which can be related to a reduced osteogenic potential of bone marrow mesenchymal stem cells (BM-MSCs). MSCs from diabetic rats (dBM-MSC) have shown a tendency to differentiate towards adipocytes (AD) instead of osteoblasts (OB). Since photobiomodulation (PBM) therapy is a non-invasive treatment capable of recovering the osteogenic potential of dBM-MSCs, we aimed to evaluate whether PBM can modulate MSC's differentiation under hyperglycemic conditions. BM-MSCs of healthy and diabetic rats were isolated and differentiated into osteoblasts (OB and dOB) and adipocytes (AD and dAD). dOB and dAD were treated with PBM every 3 days (660 nm; 5 J/cm(2); 0.14 J; 20 mW; 0.714 W/cm(2)) for 17 days. Cell morphology and viability were evaluated, and cell differentiation was confirmed by gene expression (RT-PCR) of bone (Runx2, Alp, and Opn) and adipocyte markers (Ppar gamma, C/Ebp alpha, and C/Ebp beta), production of extracellular mineralized matrix (Alizarin Red), and lipid accumulation (Oil Red). Despite no differences on cell morphology, the effect of DM on cells was confirmed by a decreased gene expression of bone markers and matrix production of dOB, and an increased expression of adipocyte and lipid accumulation of dAD, compared to heatlhy cells. On the other hand, PBM reversed the effects of dOB and dAD. The negative effect of DM on cells was confirmed, and PBM improved OB differentiation while decreasing AD differentiation, driving the fate of dBM-MSCs. These results may contribute to optimizing bone regeneration in diabetic patients. (AU)

• FAPESP's process: 18/20302-5 - The effect of photobiomodulation therapy on osteoblast differentiation of mesenchymal stem cell derived from bone marrow of diabetic rats
• Grantee: Isabella Nunes Copete
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 21/04874-1 - Priming effect of phothobiomodulation therapy on osteogenic potential of adipose tissue-derived mesenchymal stem cells
• Grantee: Emanuela Prado Ferraz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/23350-3 - The role of photobiomodulation therapy in the fate of bone marrow-derived mesenchymal stem cells in diabetic rats
• Grantee: Cecília Cardoso Kfouri
• Support Opportunities: Scholarships in Brazil - Scientific Initiation