Detection of Listeria innocua in the dairy processing chain: resistance to antibiotics and essential oils

Author(s)

Mayara Cardoso da ROSA ^[1] ; Raiza IACUZIO ^[2] ; Giovana Rueda BARBOSA ^[3] ; Rodrigo de Castro Lisboa PEREIRA ^[4] ; Melina CRUZADO-BRAVO ^[5] ; Vera Lucia Mores RALL ^[6] ; Deyse Christina VALLIM ^[7] ; Nathália Cristina Cirone SILVA ^[8]

Affiliation

^[1] Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Departamento de Agroindústria, Alimentos e Nutrição - Brasil

^[2] Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos. Departamento de Ciência de Alimentos e Nutrição - Brasil

^[3] Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos. Departamento de Ciência de Alimentos e Nutrição - Brasil

^[4] Fundação Oswaldo Cruz. Laboratório de Zoonoses Bacterianas - Brasil

^[5] Universidad Nacional Autónoma de Chota. Facultad Ciencias Agrarias. Escuela Profesional de Ingeniería Agroindustrial - Peru

^[6] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Instituto de Biociências. Departamento de Microbiologia e Imunologia - Brasil

^[7] Fundação Oswaldo Cruz. Laboratório de Zoonoses Bacterianas - Brasil

^[8] Universidade Estadual de Campinas. Faculdade de Engenharia de Alimentos. Departamento de Ciência de Alimentos e Nutrição - Brasil

• Document type: Journal article
• Source: FOOD SCIENCE AND TECHNOLOGY; v. 42, 2022-08-29.

Abstract

Abstract Listeria monocytogenes is a food pathogen responsible for many foodborne disease outbreaks. Listeria innocua is similar to L. monocytogenes, could be considered biologically similar to L. monocytogenes and could be used to predict its behavior. The aims of this work were to isolate Listeria spp. in dairy processing plants, perform genotypic characterization of virulence, evaluate the antimicrobial resistance profile, and verify the sensitivity of the strains to essential oils. Sampling was held in six dairy processing plants in the region of Campinas, Brazil. Seventeen Listeria innocua strains were isolated, all negative for virulence genes inlA, inlB, inlC, inlJ, actA, plcA, and plcB. The strains were resistant to kanamycin (5.9%), penicillin (11.8%), ampicillin (11.8%), cefoxitin (58.8%), clindamycin (76.50%) and oxacillin (100%). The susceptibility of the strains to commercial essential oils (Rosemarinus officinalis, Cymbopogon citratus, Eucalyptus citirodora, Mentha piperita, Piper nigrum, Vetiveria zizanioides, Cymbopogon nardus, Cymbopogon martinii and Pogostemon cablin) was evaluated by Minimum Inhibitory Concentration (MIC) test. The results showed Cymbopogon citratus, Cymbopogon martini, Cymbopogon nardus and Mentha piperita oils were most efficient. The resistance of Listeria innocua isolated in dairy processing plants is worrisome, and the use of essential oils could be an alternative for development of new drugs and products. (AU)

• FAPESP's process: 16/12322-0 - Isolation and characterization of Listeria monocytogenes in dairies from Campinas region
• Grantee: Mayara Cardoso da Rosa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation