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Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae

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Author(s):
Bernegossi, Agda Maria ; Vozdova, Miluse ; Cernohorska, Halina ; Kubickova, Svatava ; Galindo, David Javier ; Kadlcikova, Dita ; Rubes, Jiri ; Barbanti Duarte, Jose Mauricio
Total Authors: 8
Document type: Journal article
Source: Cytogenetic and Genome Research; v. N/A, p. 8-pg., 2022-08-18.
Abstract

Cervids are characterized by their greatest karyotypic diversity among mammals. A great diversity of chromosome numbers in notably similar morphological groups leads to the existence of several complexes of cryptic species and taxonomic uncertainties. Some deer lineages, such as those of Neotropical deer, stand out for a rapid chromosomal reorganization and intraspecific chromosome polymorphisms, which have not been properly explored yet. For that reason, we contribute to the study of deer karyotype diversity and taxonomy by producing and characterizing new molecular cytogenetic markers for the gray brocket deer (Subulo gouazoubira), a deer species that retained the hypothetical ancestral karyotype of Cervidae. We used bacterial artificial chromosome (BAC) clones derived from the cattle genome (Bos taurus) as markers, which were hybridized on S. gouazoubira metaphase chromosomes. In total, we mapped 108 markers, encompassing all gray brocket deer chromosomes, except the Y chromosome. The detailed analysis of fluorescent in situ hybridization results showed 6 fissions and 1 fusion as interchromosomal rearrangements that have separated cattle and gray brocket deer karyotypes. Each group of BAC probes derived from bovine chromosome pairs 1, 2, 5, 6, 8, and 9 showed hybridization signals on 2 different chromosomes, while pairs 28 and 26 are fused in tandem in a single acrocentric chromosome in S. gouazoubira. Furthermore, the BAC markers detected the occurrence of intrachromosomal rearrangements in the S. gouazoubira chromosomes homologous to pair 1 and the X chromosome of cattle. We present a karyotypic map of the 108 new markers, which will be of great importance for future karyotypic evolution studies in cervids and, consequently, help in their conservation and taxonomy resolution. (AU)

FAPESP's process: 17/07014-8 - The use of actual topotypes to produce genotypes and cytotypes in the taxonomic review of the Mazama genus: the basis for the species conservation
Grantee:José Maurício Barbanti Duarte
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 19/20810-3 - Characterization of homologies between bovine (Bos taurus) BAC probes and gray brocket deer (Mazama gouazoubira) chromosomes: new perspectives in intra and interspecific studies of the Mazama genus
Grantee:Agda Maria Bernegossi
Support Opportunities: Scholarships abroad - Research Internship - Master's degree
FAPESP's process: 19/06940-1 - The use of molecular cytogenetics for evolutioonary and taxonomic studies in Cervidae
Grantee:José Maurício Barbanti Duarte
Support Opportunities: Regular Research Grants
FAPESP's process: 18/25769-9 - Characterization of homologies between bovine BACs probes (Bos taurus) and gray brocket deer chromosomes (Mazama gouazoubira): new perspectives for intra and interespecific studies in the genus Mazama
Grantee:Agda Maria Bernegossi
Support Opportunities: Scholarships in Brazil - Master