Immune System Modulation by the Adjuvants Poly (I:C) and Montanide ISA 720

Author(s)

Marques, Rodolfo F. ; de Melo, Filipe Menegatti ; Novais, Janaina Tenorio ; Soares, Irene S. ; Bargieri, Daniel Youssef ; Gimenez, Alba Marina

• Document type: Journal article
• Source: FRONTIERS IN IMMUNOLOGY; v. 13, p. 14-pg., 2022-06-29.

Abstract

Adjuvants are essential for vaccine development, especially subunit-based vaccines such as those containing recombinant proteins. Increasing the knowledge of the immune response mechanisms generated by adjuvants should facilitate the formulation of vaccines in the future. The present work describes the immune phenotypes induced by Poly (I:C) and Montanide ISA 720 in the context of mice immunization with a recombinant protein based on the Plasmodium vivax circumsporozoite protein (PvCSP) sequence. Mice immunized with the recombinant protein plus Montanide ISA 720 showed an overall more robust humoral response, inducing antibodies with greater avidity to the antigen. A general trend for mixed Th1/Th2 inflammatory cytokine profile was increased in Montanide-adjuvanted mice, while a balanced profile was observed in Poly (I:C)-adjuvanted mice. Montanide ISA 720 induced a gene signature in B lymphocytes characteristic of heme biosynthesis, suggesting increased differentiation to Plasma Cells. On the other hand, Poly (I:C) provoked more perturbations in T cell transcriptome. These results extend the understanding of the modulation of specific immune responses induced by different classes of adjuvants, and could support the optimization of subunit-based vaccines. (AU)

• FAPESP's process: 12/13032-5 - Generation and analysis of the immunogenicity of recombinant proteins based on the different allelic forms of the circumsporozoite antigen of Plasmodium vivax aiming at the development of a universal vaccine against malaria
• Grantee: Irene da Silva Soares
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/25993-6 - Stability study of a recombinant protein based on Plasmodium vivax circumsporozoite protein candidate for malaria vaccine
• Grantee: Janaína Tenorio Novais
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/18102-7 - Preclinical tests of recombinant adenovirus-based protein and vaccine formulations expressing the Plasmodium vivax circumsporozoite protein
• Grantee: Alba Marina Gimenez
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/04455-9 - The use of mRNA-based technology platform as a strategy on development of a multi-antigen/multi-stage vaccine formulation against Plasmodium vivax
• Grantee: Rodolfo Ferreira Marques
• Support Opportunities: Scholarships in Brazil - Post-Doctoral