| Full text | |
| Author(s): |
Kobal, M. B.
;
Camacho, S. A.
;
Moreira, L. G.
;
Toledo, K. A.
;
Tada, D. B.
;
Aoki, P. H. B.
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Biophysical Chemistry; v. 300, p. 9-pg., 2023-09-01. |
| Abstract | |
Gold nanoparticles are valuable photothermal agents owing to their efficient photothermal conversion, photobleaching resistance, and potential surface functionalization. Herein, we combined bioinspired membranes with in vitro assays to elicit the molecular mechanisms of gold shell-isolated nanoparticles (AuSHINs) on ductal mammary carcinoma cells (BT-474). Langmuir and Langmuir-Schaefer (LS) films were handled to build biomembranes from BT-474 lipid extract. AuSHINs incorporation led to surface pressure-area (& pi;-A) isotherms expansion, increasing membrane flexibility. Fourier-transform infrared spectroscopy (FTIR) of LS multilayers revealed electrostatic AuSHINs interaction with head portions of BT-474 lipid extract, causing lipid chain disorganization. Limited AuSHINs insertion into monolayer contributed to hydroperoxidation of the unsaturated lipids upon irradiation, consistently with the surface area increments of ca. 2.0%. In fact, membrane disruption of irradiated BT-474 cells containing AuSHINs was confirmed by confocal microscopy and LDH leakage, with greater damage at 2.2 x 1013 AuSHINs/mL. Furthermore, the decrease in nuclei dimensions indicates cell death through photoinduced damage. (AU) | |
| FAPESP's process: | 18/14692-5 - Photothermal therapy mediated by gold nanoparticles: from model systems of biomembranes toward in vitro culture of tumor cells |
| Grantee: | Sabrina Aléssio Camacho |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/16713-0 - Photodynamic therapy: from molecular effects in model systems toward photodynamic efficiency in cell culture |
| Grantee: | Pedro Henrique Benites Aoki |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/14500-1 - Towards convergence of photodynamic (TFD) and photothermal (TFT) therapies on in vitro culture of melanoma-derived cells |
| Grantee: | Mirella Boaro Kobal |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 20/15854-9 - Modulating photochemical reactions in Escherichia coli lipid monolayers with the binding mechanisms of eosin decyl ester and toluidine blue-O photosensitizers |
| Grantee: | Lucas Gontijo Moreira |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/11408-3 - Multi-User Equipment approved in grant 2013/14262-7: confocal microscope |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/02189-2 - Plasmonic nanostructures for synergistic photodynamic (PDT) and photothermal (PTT) therapy of cancer cells |
| Grantee: | Pedro Henrique Benites Aoki |
| Support Opportunities: | Research Grants - Initial Project |
| FAPESP's process: | 17/03879-4 - EMU granted in the process 2013 / 14262-7 thematic project - DLS |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Multi-user Equipment Program |