Hsp90 and metal-binding J-protein family chaperones are not critically involved in cellular iron-sulfur protein assembly and iron regulation in yeast

Author(s)

Carvalho, Felipe A. ; Muehlenhoff, Ulrich ; Braymer, Joseph J. ; Root, Vasilij ; Stuempfig, Martin ; Oliveira, Carla C. ; Lill, Roland

• Document type: Journal article
• Source: FEBS Letters; v. 597, n. 13, p. 15-pg., 2023-03-30.

Abstract

Systematic studies have revealed interactions between components of the Hsp90 chaperone system and Fe/S protein biogenesis or iron regulation. In addition, two chloroplast-localized DnaJ-like proteins, DJA5 and DJA6, function as specific iron donors in plastidial Fe/S protein biogenesis. Here, we used Saccharomyces cerevisiae to study the impact of both the Hsp90 chaperone and the yeast DJA5-DJA6 homologs, the essential cytosolic Ydj1, and the mitochondrial Mdj1, on cellular iron-related processes. Despite severe phenotypes induced upon depletion of these crucial proteins, there was no critical in vivo impact on Fe/S protein biogenesis or iron regulation. Importantly, unlike the plant DJA5-DJA6 iron chaperones, Ydj1 and Mdj1 did not bind iron in vivo, suggesting that these proteins use zinc for function under normal physiological conditions. (AU)

• FAPESP's process: 19/00527-5 - Characterization of the role of Nop17, R2TP complex-member, in the cytosolic Fe-S protein assembly (CIA) system in Saccharomyces cerevisiae
• Grantee: Felipe Aguilar Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/00901-1 - Posttranscriptional control of gene expression: pre-rRNA processing, mRNA degradation, splicing and snoRNP assembly in Saccharomyces cerevisiae
• Grantee: Carla Columbano de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/06497-0 - Characterization of the role of Nop17, r2tp-complex member, in the cytosolic iron-sulfur protein assembly (CIA) system in Saccharomyces cerevisiae
• Grantee: Felipe Aguilar Carvalho
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)