Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil

Author(s)

Campioni, Fabio ; Gomes, Carolina Nogueira ; Rodrigues, Dalia dos Prazeres ; Bergamini, Alzira Maria Morato ; Falcao, Juliana Pfrimer

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 52, n. 1, p. 11-pg., 2020-10-26.

Abstract

Salmonella Enteritidis has caused, since the 1980s, a sustained epidemic of human infections in many countries. This study analyzed S. Enteritidis strains isolated before and after the epidemic period in Brazil regarding their capacities to survive to acid, oxidative, and high-temperature stresses, and capacity to grow in egg albumen. Moreover, the ability to invade human epithelial cells (Caco-2) and to survive inside human (U937) and chicken (HD11) macrophages was checked. Post-epidemic strains showed a better ability to survive after 10 min under acid stress at 37 degrees C (P <= 0.05). However, both groups of strains showed similar ability to survive after 1 h under acid stress at 37 degrees C and at 42 degrees C independently of the time of exposure. Similar ability was verified in both groups of strains regarding oxidative stress, growth in egg albumen, high-temperature stress, invasion to Caco-2 cells, and invasion and survival in macrophages. In conclusion, post-epidemic S. Enteritidis strains showed a better ability to survive under the acid stress found in the stomach, which might be an advantage to reach the intestine and colonize chickens and humans. However, both groups of strains did not differ significantly in the majority of the phenotypic tests analyzed in this study. (AU)

• FAPESP's process: 13/25191-3 - Phenotypical analysis and transcriptome and genome sequencing in Salmonella Enteritidis strains isolated in the pre and post-pandemic period in Brazil
• Grantee: Fábio Campioni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/24716-3 - Sequencing of the Genome, transcriptoma and phenotypic analysis of Campylobacter coli strains isolated from different sources in Brazil
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants