Evidence Linking PPARG Genetic Variants with Periodontitis and Type 2 Diabetes Mellitus in a Brazilian Population

Author(s)

Cirelli, Thamiris ; Nicchio, Ingra G. ; Bussaneli, Diego G. ; Silva, Barbara R. ; Nepomuceno, Rafael ; Orrico, Silvana R. P. ; Cirelli, Joni A. ; Theodoro, Leticia H. ; Barros, Silvana P. ; Scarel-Caminaga, Raquel M.

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 7, p. 17-pg., 2023-04-01.

Abstract

The peroxisome proliferator-activated receptor gamma (PPARG) gene encodes a transcription factor involved in the regulation of complex metabolic and inflammatory diseases. We investigated whether single nucleotide polymorphisms (SNPs) and haplotypes of the PPARG gene could contribute with susceptibility to develop periodontitis alone or together with type 2 diabetes mellitus (T2DM). Moreover, we evaluated the gene-phenotype association by assessing the subjects' biochemical and periodontal parameters, and the expression of PPARG and other immune response-related genes. We examined 345 subjects with a healthy periodontium and without T2DM, 349 subjects with moderate or severe periodontitis but without T2DM, and 202 subjects with moderate or severe periodontitis and T2DM. PPARG SNPs rs12495364, rs1801282, rs1373640, and rs1151999 were investigated. Multiple logistic regressions adjusted for age, sex, and smoking status showed that individuals carrying rs1151999-GG had a 64% lower chance of developing periodontitis together with T2DM. The CCGT haplotype increased the risk of developing periodontitis together with T2DM. The rs1151999-GG and rs12495364-TC were associated with reduced risk of obesity, periodontitis, elevated triglycerides, and elevated glycated hemoglobin, but there was no association with gene expression. Polymorphisms of the PPARG gene were associated with developing periodontitis together with T2DM, and with obesity, lipid, glycemic, and periodontal characteristics. (AU)

• FAPESP's process: 16/08070-6 - Investigation of genetic susceptibility to chronic periodontitis associated with Type 2 Diabetes mellitus
• Grantee: Thamiris Cirelli
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 16/03753-8 - Investigation of potential genetic markers of chronic periodontitis associated or not with type 2 diabetes mellitus through patient's genetic susceptibility and both glycemic and lipid profiles
• Grantee: Raquel Mantuaneli Scarel Caminaga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/13295-1 - Investigation of the association of genetic variation regarding susceptibility to chronic periodontal disease by OpenArray
• Grantee: Rafael Nepomuceno Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/18313-3 - Investigation of genetic variations susceptibility to chronic periodontitis by OpenArray®: Biostatistics, Bioinformatics and Functional Analysis
• Grantee: Rafael Nepomuceno Oliveira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/26367-1 - Investigation of polymorphs in the DEFB1 gene with susceptibility to chronic periodontal disease and type 2 diabetes mellitus
• Grantee: Ana Cláudia dos Santos Rios
• Support Opportunities: Scholarships in Brazil - Scientific Initiation