Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats

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Sergio Gushiken, Lucas Fernando ; Hussni, Carlos Alberto ; Bastos, Jairo Kenupp ; Rozza, Ariane Leite ; Beserra, Fernando Pereira ; Vieira, Ana Julia ; Padovani, Carlos Roberto ; Lemos, Marivane ; Polizello Junior, Maurilio ; Mangabeira da Silva, Jonas Joaquim ; Nobrega, Rafael Henrique ; Monteiro Martinez, Emanuel Ricardo ; Pellizzon, Claudia Helena

• Document type: Journal article
• Source: Evidence-based Complementary and Alternative Medicine; v. 2017, p. 16-pg., 2017-01-01.

Abstract

The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n = 8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/23684-5 - Evaluation of cellular and molecular mechanisms in skin wound healing using Copaifera langsdorffii hydroalcoholic extract and oil resin ointments
• Grantee: Lucas Fernando Sérgio Gushiken
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/15092-5 - This project is integrant of chemical and pharmacological validation of extracts and active compounds of copaifera speci
• Grantee: Lucas Fernando Sérgio Gushiken
• Support Opportunities: Scholarships in Brazil - Scientific Initiation