¹³C NMR as an analytical tool for the detection of carbonic acid and pK_a determination

Author(s)

Oliveira, Daniel Rossado ; da Costa, Eric Tavares ; Schenberg, Leonardo Araujo ; Ducati, Lucas Colucci ; do Lago, Claudimir Lucio

• Document type: Journal article
• Source: Magnetic Resonance in Chemistry; v. 62, n. 2, p. 7-pg., 2024-01-23.

Abstract

NMR spectroscopy has become a standard technique in studies both on carbon capture and storage. C-13 NMR allows the detection of two peaks for carbonated aqueous samples: one for CO2(aq) and another one for the species H2CO3, HCO3-, and CO32--herein collectively named HxCO3x-2. The chemical shift of this second peak depends on the molar fraction of the three species in equilibrium and has been used to assess the equilibrium between HCO3- and CO32-. The detection of H2CO3 at low pH solutions is hindered, because of the concurrent liberation of CO2 when the medium is acidified. Herein, a valved NMR tube facilitates the detection of the HxCO3x-2 peak across a wide pH range, even at pH 1.8 where the dominant species is H2CO3. The method employed the formation of frozen layers of (NaHCO3)-C-13 and acid solutions within the tube, which are mixed as the tube reaches room temperature. At this point, the tube is already securely sealed, preventing any loss of CO2 to the atmosphere. A spectrophotometry approach allowed the measurement of the actual pH inside the pressurized NMR tube. The chemical shift for H2CO3 was determined as 160.33 +/- 0.03 ppm, which is in good agreement with value obtained by DFT calculations combined with Car-Parrinello molecular dynamics. The H2CO3 pK(a) value determined by the present method was 3.41 +/- 0.03, for 15% D2O aqueous medium and 0.8 mol/L ionic strength. The proposed method can be extended to studies about analogs such as alkyl carbonic and carbamic acids. (AU)

• FAPESP's process: 18/13960-6 - Development of instrumentation for the detection and characterization of hemiesters of carbonic acid
• Grantee: Eric Tavares da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/18727-0 - Study of 199Hg organometallic compounds in solution by ab initio molecular dynamics and relativistic NMR calculations
• Grantee: Leonardo Araujo Schenberg
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/13137-5 - Formation and properties of hemiesters of carbonic acid in aqueous medium
• Grantee: Claudimir Lucio Do Lago
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/17750-3 - Solvent Effect in NMR Parameters by ab initio Molecular Dynamics
• Grantee: Lucas Colucci Ducati
• Support Opportunities: Regular Research Grants