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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Toxoplasma gondii isolates: Multi locus RFLP-PCR genotyping from human patients in Sao Paulo State, Brazil identified distinct genotypes

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Author(s):
Ribeiro Ferreira, Isabelle Martins [1] ; Vidal, Jose Ernesto [2] ; Brandao de Mattos, Cinara de Cassia [3] ; de Mattos, Luiz Carlos [3] ; Qu, Daofeng [4] ; Su, Chunlei [5] ; Pereira-Chioccola, Vera Lucia [1]
Total Authors: 7
Affiliation:
[1] Inst Adolfo Lutz Registro, Parasitol Lab, BR-01246902 Sao Paulo - Brazil
[2] Univ Sao Paulo, Serv Extensao Ao Atendimento Pacientes HIV AIDS, Div Molestias Infecciosas & Parasitarias, Hosp Clin, Fac Med, BR-05508 Sao Paulo - Brazil
[3] Fac Med Sao Jose do Rio Preto, Lab Imunogenet, Sao Jose Do Rio Preto, SP - Brazil
[4] Zhejiang Univ, Inst Prevent Vet Med, Coll Anim Sci, Hangzhou, Zhejiang - Peoples R China
[5] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 - USA
Total Affiliations: 5
Document type: Journal article
Source: Experimental Parasitology; v. 129, n. 2, p. 190-195, OCT 2011.
Web of Science Citations: 41
Abstract

This study investigated the genetic characteristics of Toxoplasma gondii samples collected from 62 patients with toxoplasmosis in Sao Paulo State, Brazil. DNA samples were isolated from blood, cerebrospinal fluid and amniotic fluids of 25 patients with cerebral toxoplasmosis and AIDS, two patients with acute toxoplasmosis, 12 patients with ocular toxoplasmosis, six newborns with congenital toxoplasmosis and 17 pregnant women with acute infection. Diagnosis of toxoplasmosis was based in clinical, radiological and laboratory features. Genotyping was performed using multilocus PCR-RFLP genetic markers including SAG1, SAG2, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, C22-8, c29-2, L358, PK1 and Apico. Among the 62 clinical samples, 20 (32%) were successfully genotyped at eight or more genetic loci and were grouped to three distinct genotypes. Eighteen samples belonged to ToxoDB Genotype \#65 and the other two samples were identified as ToxoDB Genotypes \#6 and \#71, respectively (http://toxodb.org/toxo/). Patients presenting Genotypes \#6 and \#71 had severe and atypical cerebral toxoplasmosis, characterized by diffuse encephalitis without extensive brain lesions. These results indicate that T. gondii Genotype \#65 may have a high frequency in causing human toxoplasmosis in Sao Paulo State, Brazil. This unusual finding highlights the need to investigate the possible association of parasite genotypes with human toxoplasmosis. (C) 2011 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 08/09311-0 - Laboratorial diagnosis of toxoplasmosis focusing on congenital and cerebral toxoplasmosis
Grantee:Vera Lúcia Pereira Chioccola
Support Opportunities: Regular Research Grants
FAPESP's process: 09/05505-8 - Toxoplasma gondii polymorphic strains genotyping from patients with toxoplasmosis
Grantee:Isabelle Martins Ribeiro Ferreira
Support Opportunities: Scholarships in Brazil - Doctorate