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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of different resistance-training regimens on the WNT-signaling pathway

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Author(s):
Leal, Marcelo Larciprete [1] ; Lamas, Leonardo [2] ; Aoki, Marcelo Saldanha [3] ; Ugrinowitsch, Carlos [2] ; Carneiro Ramos, Marcela Sorelli [1] ; Tricoli, Valmor [2] ; Moriscot, Anselmo Sigari [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Phys Educ & Sport, BR-05508900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY; v. 111, n. 10, SI, p. 2535-2545, OCT 2011.
Web of Science Citations: 12
Abstract

The purpose of the present study was to evaluate the effects of 8 weeks of strength and power training on the expression of genes related to the canonical WNT pathway and beta-catenin protein levels in physically active men. Twenty-five subjects (27.4 +/- A 4.6 years) were balanced based on their relative maximum strength in the squat exercise (squat 1RM/body mass) and randomly assigned to strength training (ST) (n = 10), power training (PT) (n = 10), and control (C) (n = 5) groups. The ST and the PT groups performed high and low intensity squats, respectively, thrice a week, for 8 weeks. Muscle biopsies from the vastus lateralis muscle were collected before and after the training period. Relative strength and power increased similarly in both ST and PT groups (P < 0.001). Fiber cross-sectional area also increased similarly in both ST and PT groups. Gene expression and beta-catenin protein expression levels were assessed by real-time PCR and Western blot. Certain genes were up-regulated in the ST group (WNT1: 6.4-fold, P < 0.0001; SFRP1: 3.3-fold, P < 0.0001 and LEF1: 7.3-fold, P < 0.0001) and also in the PT group (WNT1: 24.9-fold, P < 0.0001; SFRP1: 2.7-fold, P < 0.0001; LEF1: 34.1-fold, P < 0.0001 and Cyclin D1: 7.7-fold, P < 0.001). In addition, the expression of key WNT pathway genes was substantially more responsive to PT than to ST (WNT1: P < 0.0001; LEF1: P < 0.0001 and Cyclin D1: P < 0.001). Finally, the total beta-catenin protein content increased only in the PT group (P < 0.05). Our data indicate that a PT regimen triggers greater responses in key elements of the WNT pathway. (AU)

FAPESP's process: 06/61523-7 - Cellular and molecular aspects of muscular plasticity
Grantee:Anselmo Sigari Moriscot
Support type: Research Projects - Thematic Grants
FAPESP's process: 06/00302-3 - Strength training with vibration: analysis of the determinant factors of vertical jump performance improvement
Grantee:Carlos Ugrinowitsch
Support type: Regular Research Grants