| Full text | |
| Author(s): |
T.M. Cunha
[1]
;
W.A. Verri Jr.
[2]
;
G.G. Vivancos
[3]
;
I.F. Moreira
[4]
;
S. Reis
[5]
;
C.A. Parada
[6]
;
F.Q. Cunha
[7]
;
S.H. Ferreira
[8]
Total Authors: 8
|
| Affiliation: | [1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[4] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[5] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[6] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[7] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
[8] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia - Brasil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Medical and Biological Research; v. 37, n. 3, p. 401-407, 2004-03-00. |
| Field of knowledge: | Biological Sciences - Pharmacology |
| Abstract | |
The aim of the present investigation was to describe and validate an electronic mechanical test for quantification of the intensity of inflammatory nociception in mice. The electronic pressure-meter test consists of inducing the animal hindpaw flexion reflex by poking the plantar region with a polypropylene pipette tip adapted to a hand-held force transducer. This method was compared to the classical von Frey filaments test in which pressure intensity is automatically recorded after the nociceptive hindpaw flexion reflex. The electronic pressure-meter and the von Frey filaments were used to detect time versus treatment interactions of carrageenin-induced hypernociception. In two separate experiments, the electronic pressure-meter was more sensitive than the von Frey filaments for the detection of the increase in nociception (hypernociception) induced by small doses of carrageenin (30 µg). The electronic pressure-meter detected the antinociceptive effect of non-steroidal drugs in a dose-dependent manner. Indomethacin administered intraperitoneally (1.8-15 mg/kg) or intraplantarly (30-300 µg/paw) prevented the hypersensitive effect of carrageenin (100 µg/paw). The electronic pressure-meter also detected the hypernociceptive effect of prostaglandin E2 (PGE2; 10-100 ng) in a dose-dependent manner. The hypernociceptive effect of PGE2 (100 ng) was blocked by dipyrone (160 and 320 µg/paw) but not by intraplantar administration of indomethacin (300 µg/paw). The present results validate the use of the electronic pressure-meter as more sensitive than the von Frey filaments in mice. Furthermore, it is an objective and quantitative nociceptive test for the evaluation of the peripheral antinociceptive effect of anti-inflammatory analgesic drugs, which inhibit prostaglandin synthesis (indomethacin) or directly block the ongoing hypernociception (dipyrone). (AU) | |