Improvement of a PCR test to diagnose infection by Mansonella ozzardi

Author(s)

Souza Vera, Luana Janaina ^[1] ; Basano, Sergio de Almeida ^{[1, 2]} ; Almeida Aranha Camargo, Juliana de Souza ^[1] ; de Franca, Andonai Krauze ^[3] ; Mattos Ferreira, Ricardo de Godoi ^[4] ; Casseb, Almeida Andrade ^[3] ; Medeiros, Jansen Fernandes ^{[5, 6]} ; Fontes, Gilberto ^[7] ; Aranha Camargo, Luis Marcelo ^{[1, 8]}

Affiliation

^[1] Fac Sao Lucas, Porto Velho, RO - Brazil

^[2] Ctr Med Trop Rondonia, Setor Isolamento, Porto Velho, RO - Brazil

^[3] Inst Pesquisa Patologias Tropicais, Genet Lab, Porto Velho, RO - Brazil

^[4] Fundacao Oswaldo Cruz Noroeste, Lab Estudos Bioinformat & Bioestat, Porto Velho, RO - Brazil

^[5] Inst Nacl de Pesquisas da Amazonia, Manaus, Amazonas - Brazil

^[6] Univ Estadual Amazonas, Escola Super Ciencias Saude, Manaus, Amazonas - Brazil

^[7] Univ Fed Sao Joao Rei, Ctr Ciencias Saude, Divinopolis, MG - Brazil

^[8] Inst Ciencias Biomed 5, Dept Parasitol, Monte Negro, RO - Brazil

• Document type: Journal article
• Source: Revista da Sociedade Brasileira de Medicina Tropical; v. 44, n. 3, p. 380-382, MAY-JUN 2011.
• Web of Science Citations: 12

Abstract

INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears. (AU)

• FAPESP's process: 08/10817-6 - Clinical trial with ivermectine for the treatment of Mansonella ozzardi infections
• Grantee: Luís Marcelo Aranha Camargo
• Support Opportunities: Regular Research Grants