Differential expression of apoptosis-related genes from death receptor pathway in chronic myeloproliferative diseases

Author(s):Show less -

Tognon, Raquel ; Lino Gasparotto, Elainy Patricia ; Gisele Leroy, Janine Marie ^[1] ; Vilela Oliveira, Gislane Lelis ; Neves, Renata Peres ; Viu Carrara, Rita de Cassia ^[2] ; Kashima, Simone ^[2] ; Covas, Dimas Tadeu ^[2] ; Santana, Mary ^[3] ; Souto, Elizabeth Xisto ^[3] ; Zanichelli, Maria Aparecida ^[3] ; Engel Velano, Carlos Eduardo ^[4] ; Simoes, Belinda Pinto ^[4] ; Alberto, Fernando Lopes ^[5] ; Miyashiro, Kozue ^[5] ; de Souza, Ana Maria ; Amarante-Mendes, Gustavo Pessini ^[1] ; de Castro, Fabiola Attie ^[6]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[2] USP, Hosp Clin, Ribeirao Preto Sch Med, Ribeirao Preto Ctr Transfus Med, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Brigadeiro Hosp Sao Paulo, Sao Paulo - Brazil

^[4] USP, Ribeirao Preto Sch Med, Dept Clin Med, BR-14040903 Ribeirao Preto, SP - Brazil

^[5] Fleury Med & Hlth, Sao Paulo - Brazil

^[6] USP, Fac Ciencias Farmaceut, Hematol Lab, Ribeirao Preto Sch Pharmaceut Sci, Dept Clin Toxicol & Bromatol Anal, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of Clinical Pathology; v. 64, n. 1, p. 75-82, JAN 2011.
• Web of Science Citations: 18

Abstract

Background Chronic myeloproliferative disorders (MPDs) are clonal haematopoietic stem cell malignancies characterised by an accumulation of mature myeloid cells in bone marrow and peripheral blood. Deregulation of the apoptotic machinery may be associated with MPD physiopathology. Aims To evaluate expression of death receptors' family members, mononuclear cell apoptosis resistance, and JAK2 allele burden. Subjects and Methods Bone marrow haematopoietic progenitor CD34 cells were separated using the Ficoll-hypaque protocol followed by the Miltenyi CD34 isolation kit, and peripheral blood leukocytes were separated by the Haes-Steril method. Total RNA was extracted by the Trizol method, the High Capacity Kit was used to synthesise cDNA, and real-time PCR was performed using SybrGreen in ABIPrism 7500 equipment. The results of gene expression quantification are given as 2(-Delta Delta Ct). The JAK2 V617F mutation was detected by real-time allelic discrimination PCR assay. Peripheral blood mononuclear cells (PBMCs) were isolated by the Ficoll-hypaque protocol and cultured in the presence of apoptosis inducers. Results In CD34 cells, there was mRNA overexpression for fas, faim and c-flip in polycythaemia vera (PV), essential thrombocythaemia (ET) and primary myelofibrosis (PMF), as well as fasl in PMF, and dr4 levels were increased in ET. In leukocytes, fas, c-flip and trail levels were increased in PV, and dr5 expression was decreased in ET. There was an association between dr5 and fasl expression and JAK2V617F mutation. PBMCs from patients with PV, ET or PMF showed resistance to apoptosis inducers. Conclusions The results indicate deregulation of apoptosis gene expression, which may be associated with MPD pathogenesis leading to accumulation of myeloid cells in MPDs. (AU)