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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Recession versus Tenotomy Surgery without Recession in Adult Rabbit Extraocular Muscle

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Author(s):
Christiansen, Stephen P. [1, 2] ; Antunes-Foschini, Rosalia S. [3] ; McLoon, Linda K. [4, 5]
Total Authors: 3
Affiliation:
[1] Boston Univ, Sch Med, Dept Ophthalmol, Boston, MA 02118 - USA
[2] Boston Univ, Sch Med, Dept Pediat, Boston, MA 02118 - USA
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Ophthalmol, Ribeirao Preto - Brazil
[4] Univ Minnesota, Dept Ophthalmol, Minneapolis, MN 55455 - USA
[5] Univ Minnesota, Dept Neurosci, Minneapolis, MN 55455 - USA
Total Affiliations: 5
Document type: Journal article
Source: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE; v. 51, n. 11, p. 5646-5656, NOV 2010.
Web of Science Citations: 15
Abstract

PURPOSE. Surgical recession of an extraocular muscle (EOM) posterior to its original insertion is a common form of strabismus surgery, weakening the rotational force exerted by the muscle on the globe and improving eye alignment. The purpose of this study was to assess myosin heavy chain (MyHC) isoform expression and satellite cell activity as defined by Pax7 expression in recessed EOMs of adult rabbits compared with that in muscles tenotomized but not recessed and with that in normal control muscles. METHODS. The scleral insertion of the superior rectus muscle was detached and sutured either 7 mm posterior to its original insertion site (recession surgery) or at the same site (tenotomy). One day before euthanatization, the rabbits received bromodeoxyuridine (BrdU) injections. After 7 and 14 days, selected EOMs from both orbits were examined for changes in fast, slow, neonatal, and developmental MyHC isoform expression, Pax7 expression, and BrdU incorporation. RESULTS. Recession and tenotomy surgery resulted in similar changes in the surgical EOMs. These included a decreased proportion of fast MyHC myofibers, an increased proportion of slow MyHC myofibers, and increased BrdU-positive satellite cells. Similar changes were seen in the non-operated contralateral superior rectus muscles. The ipsilateral inferior rectus showed reciprocal changes to the surgical superior rectus muscles. CONCLUSIONS. The EOMs are extremely adaptive to changes induced by recession and tenotomy surgery, responding with modulations in fiber remodeling and myosin expression. These adaptive responses could be manipulated to improve surgical success rates. (Invest Ophthalmol Vis Sci. 2010;51:5646-5656) DOI:10.1167/iovs.10-5523 (AU)

FAPESP's process: 07/05260-0 - Study of the process of satellite cells activation in rabbit extraocular muscles
Grantee:Rosália Maria Simões AntunesFoschini
Support Opportunities: Scholarships abroad - Research