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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Purification and Characterization of the alpha-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756

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Author(s):
Azevedo Carvalho, Ana Flavia [1] ; Boscolo, Mauricio [1] ; da Silva, Roberto [1] ; Ferreira, Henrique [1] ; Gomes, Eleni [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ Unesp, Lab Bichem & Appl Microbiol, BR-15054000 Sao Jose Do Rio Preto - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF MICROBIOLOGY; v. 48, n. 4, p. 452-459, AUG 2010.
Field of knowledge: Biological Sciences - Microbiology
Web of Science Citations: 4
Abstract

An alpha-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose 12 columns. The apparent molecular mass of the enzyme was 83 kDa as determined in gel electrophoresis. Maximum activity was observed at pH 4.5 at 70 degrees C. Enzyme showed stability stable in the pH range of 3.0-9.0 and lost 40% of its initial activity at the temperatures of 40, 50, and 60 C. In the presence of ions Na(+), Ba(2+), Co(2+), Ni(2+), Mg(2+), Mn(2+), Al(3+), Zn(2+), Ca(2+) this enzyme maintained 90-105% of its maximum activity and was inhibited by Cr(3+), Ag(+), and Hg(2+). The enzyme showed a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides and alpha-PNPG. The K(m) measured for the a-glucosidase was 0.07 mu M, with a V(max) of 318.0 mu mol/min/mg. (AU)

FAPESP's process: 10/12624-0 - Application of physical-chemical and enzymatic methods in the saccharification of sugar cane bagasse: studies on microorganisms, fermentative processes and hydrolyses methods
Grantee:Eleni Gomes
Support type: Research Projects - Thematic Grants