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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MuRF1 is a muscle fiber-type II associated factor and together with MuRF2 regulates type-II fiber trophicity and maintenance

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Author(s):
Moriscot, Anselmo S. [1] ; Baptista, Igor L. [1, 2] ; Bogomolovas, Julius [2] ; Witt, Christian [2] ; Hirner, Stephanie [2] ; Granzier, Henk [3] ; Labeit, Siegfried [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Med Mannheim, Inst Integrat Pathophysiol, D-68167 Mannheim - Germany
[3] Univ Arizona, Dept Physiol, Tucson, AZ 85724 - USA
Total Affiliations: 3
Document type: Journal article
Source: Journal of Structural Biology; v. 170, n. 2, SI, p. 344-353, MAY 2010.
Web of Science Citations: 47
Abstract

MuRF1 is a member of the RBCC (RING, B-box, coiled-coil) superfamily that has been proposed to act as an atrogin during muscle wasting. Here, we show that MuRF1 is preferentially induced in type-II muscle fibers after denervation. Fourteen days after denervation, MuRF1 protein was further elevated but remained preferentially expressed in type-II muscle fibers. Consistent with a fiber-type dependent function of MuRF1, the tibialis anterior muscle (rich in type-II muscle fibers) was considerably more protected in MuRF1-KO mice from muscle wasting when compared to soleus muscle with mixed fiber-types. We also determined fiber-type distributions in MuRF1/MuRF2 double-deficient KO (dKO) mice, because MuRF2 is a close homolog of MuRF1. MuRF1/MuRF2 dKO mice showed a profound loss of type-II fibers in soleus muscle. As a potential mechanism we identified the interaction of MuRF1/MuRF2 with myozenin-1, a calcineurin/NFAT regulator and a factor required for maintenance of type-II muscle fibers. MuRF1/MuRF2 dKO mice had lost myozenin-1 expression in tibialis anterior muscle, implicating MuRF1/MuRF2 as regulators of the calcineurin/NFAT pathway. In summary, our data suggest that expression of MuRF1 is required for remodeling of type-II fibers under pathophysiological stress states, whereas MuRF1 and MuRF2 together are required for maintenance of type-II fibers, possibly via the regulation of myozenin-1. (C) 2010 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 06/61523-7 - Cellular and molecular aspects of muscular plasticity
Grantee:Anselmo Sigari Moriscot
Support type: Research Projects - Thematic Grants