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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of metallo-beta-lactamases-encoding genes in environmental isolates of Aeromonas hydrophila and Aeromonas jandaei

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Author(s):
Balsalobre, L. C. [1] ; Dropa, M. [1] ; Lincopan, N. [2, 3] ; Mamizuka, E. M. [2] ; Matte, G. R. [1] ; Matte, M. H. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Publ Hlth, Publ Hlth Lab, BR-01246904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, BR-01246904 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-01246904 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Letters in Applied Microbiology; v. 49, n. 1, p. 142-145, JUL 2009.
Web of Science Citations: 13
Abstract

Aims: To determine the prevalence and expression of metallo-beta-lactamases (MBL)-encoding genes in Aeromonas species recovered from natural water reservoirs in southeastern Brazil. Methods and Results: Eighty-seven Aeromonas isolates belonging to Aeromonas hydrophila (n = 41) and Aer. jandaei (n = 46) species were tested for MBL production by the combined disk test using imipenem and meropenem disks as substrates and EDTA or thioglycolic acid as inhibitors. The presence of MBL genes was investigated by PCR and sequencing using new consensus primer pairs designed in this study. The cphA gene was found in 97.6% and 100% of Aer. hydrophila and Aer. jandaei isolates, respectively, whereas the acquired MBL genes bla(IMP), bla(VIM) and bla(SPM-1) were not detected. On the other hand, production of MBL activity was detectable in 87.8% and 10.9% of the cphA-positive Aer. hydrophila and Aer. jandaei isolates respectively. Conclusions: Our results indicate that cphA seems to be intrinsic in the environmental isolates of Aer. hydrophila and Aer. jandaei in southeastern Brazil, although, based on the combined disk test, not all of them are apparently able to express the enzymatic activity. Significance and Impact of the Study: These data confirm the presence of MBL-producing Aeromonas species in natural water reservoirs. Risk of water-borne diseases owing to domestic and industrial uses of freshwater should be re-examined from the increase of bacterial resistance point of view. (AU)

FAPESP's process: 07/02266-7 - Ocurrence of virulence and resistance genes in Aeromonas hydrophila and Aeromonas jandaei from environmental samples
Grantee:Livia Carminato Balsalobre
Support Opportunities: Scholarships in Brazil - Master