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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Na plus -Glucose Cotransporter SGLT1 Protein in Salivary Glands: Potential Involvement in the Diabetes-Induced Decrease in Salivary Flow

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Sabino-Silva, R. [1] ; Freitas, H. S. [1] ; Lamers, M. L. [2] ; Okamoto, M. M. [1] ; Santos, M. F. [2] ; Machado, U. F. [1]
Total Authors: 6
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05505900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05505900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Membrane Biology; v. 228, n. 2, p. 63-69, MAR 2009.
Web of Science Citations: 24

Oral health complications in diabetes include decreased salivary secretion. The SLC5A1 gene encodes the Na(+)-glucose cotransporter SGLT1 protein, which not only transports glucose, but also acts as a water channel. Since SLC5A1 expression is altered in kidneys of diabetic subjects, we hypothesize that it could also be altered in salivary glands, contributing to diabetic dysfunction. The present study shows a diabetes-induced decrease (p < 0.001) in salivary secretion, which was accompanied by enhanced (p < 0.05) SGLT1 mRNA expression in parotid (50%) and submandibular (30%) glands. Immunohistochemical analysis of parotid gland of diabetic rats revealed that SGLT1 protein expression increased in the luminal membrane of ductal cells, which can stimulate water reabsorption from primary saliva. Furthermore, SGLT1 protein was reduced in myoepithelial cells of the parotid from diabetic animals, and that, by reducing cellular contractile activity, might also be related to reduced salivary flux. Six-day insulin-treated diabetic rats reversed all alterations. In conclusion, diabetes increases SLC5A1 gene expression in salivary glands, increasing the SGLT1 protein content in the luminal membrane of ductal cells, which, by increasing water reabsorption, might explain the diabetes-induced decrease in salivary secretion. (AU)

FAPESP's process: 07/50554-1 - Glucose transporters and diabetes mellitus: contribution to the knowledge of glycemic control and chronic diseases development
Grantee:Ubiratan Fabres Machado
Support type: Research Projects - Thematic Grants