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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antiprotozoan activity of Brazilian marine cnidarian extracts and of a modified steroid from the octocoral Carijoa riisei

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Author(s):
Reimao, Juliana Quero [1] ; Migotto, Alvaro Esteves [2] ; Kossuga, Miriam H. [3] ; Berlinck, Roberto G. S. [3] ; Tempone, Andre Gustavo [1]
Total Authors: 5
Affiliation:
[1] Inst Adolfo Lutz Registro, Dept Parasitol, Lab Toxinol Aplicada, BR-01246000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ctr Biol Marinha, BR-11600000 Sao Sebastiao, SP - Brazil
[3] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Parasitology Research; v. 103, n. 6, p. 1445-1450, NOV 2008.
Web of Science Citations: 26
Abstract

In the present investigation, we have evaluated the antileishmanial and antitrypanosomal activity of methanolic crude extracts obtained from eight species of cnidarians and of a modified steroid isolated from the octocoral Carijoa riisei. The antileishmanial activity of cnidarians crude extracts showed 50% inhibitory concentration ( IC50) values in the concentration range between 2.8 and 93.3 mu g/mL. Trypomastigotes of Trypanosoma cruzi were less susceptible to the crude extracts, with IC50 values in the concentration range between 40.9 and 117.9 mu g/mL. The steroid (18-acetoxipregna-1,4,20-trien-3-one) displayed a strong antileishmanial activity, with an IC50 value of 5.5 mu g/mL against promastigotes and 16.88 mu g/mL against intracellular amastigotes. The steroid also displayed mammalian cytotoxicity (IC50 of 10.6 mu g/mL), but no hemolytic activity was observed at the highest concentration of 12.5 mu g/mL. The antileishmanial effect of the steroid in macrophages suggested other mechanism than macrophage activation, as no upregulation of nitric oxide was observed. The antitrypanosomal activity of the steroid resulted in an IC50 value of 50.5 mu g/mL. These results indicate the potential of cnidarian natural compounds as antileishmanial drug candidates. (AU)

FAPESP's process: 06/05241-2 - Study of anti-Leishmania activity of Brazilian marine invertebrate compounds
Grantee:Juliana Quero Reimão Dalla Zanna
Support type: Scholarships in Brazil - Master
FAPESP's process: 05/60175-2 - Discovery and development of potential chemotherapeutic agents based on marine invertebrates and associated micro-organisms
Grantee:Roberto Gomes de Souza Berlinck
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 05/00974-9 - Evaluation of the therapeutic potential of amphibian and snake venoms in Leishmaniasis and Chagas' Disease
Grantee:André Gustavo Tempone Cardoso
Support type: Regular Research Grants