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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modulation of cutaneous inflammation induced by ticks in contrasting phenotypes of infestation in bovines

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Author(s):
Carvalho, Wanessa Araujo ; Franzin, Alessandra Mara ; Rodrigues Abatepaulo, Antonio Roberto ; Freire de Oliveira, Carlo Jose ; More, Daniela Dantas ; da Silva, Joao Santana ; Ferreira, Beatriz Rossetti [1] ; Ferreira de Miranda Santos, Isabel K. [2, 3]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Maternal & Child & Publ Hlth Nursing, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Embrapa Recursos Genet & Biotecnol, BR-70770900 Brasilia, DF - Brazil
[3] Univ Sao Paulo, Depto Bioquim & Imunol, Fac Med Ribeirao Preto, Ribeirao Preto Sch Med, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Veterinary Parasitology; v. 167, n. 2-4, SI, p. 260-273, FEB 10 2010.
Web of Science Citations: 22
Abstract

Tick saliva contains molecules that are inoculated at the site of attachment on their hosts in order to modulate local immune responses and facilitate a successful blood meal. Bovines express heritable, contrasting phenotypes of infestations with the cattle tick, Rhipicephalus (Boophilus) microplus: breeds of Bos taurus indicus are significantly note resistant than those of Bos taurus taurus. Tick saliva may contain molecules that interfere with adhesion of leukocytes to endothelium and resistant hosts may mount an inflammatory profile that is more efficient to hamper the tick's blood meal. We show in vitro that adhesion of peripheral blood mononuclear cells to monolayers of cytokine-activated bovine umbilical endothelial cells was significantly inhibited by tick saliva. The inflammatory response to bites of adults of R. microplus mounted by genetically resistant and susceptible bovine hosts managed in the same pasture was investigated in vivo. The inflammatory infiltrates and levels of message coding for adhesion molecules were measured in biopsies of tick-bitten and control skin taken when animals of both breeds were exposed to low and high tick infestations. Histological studies reveal that cutaneous reactions of resistant hosts to bites of adult ticks contained significantly more basophils and eosinophils compared with reactions of the susceptible breed. Expression of the adhesion molecules - intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and P-selectin - was higher in adult-infested skin of susceptible hosts undergoing low infestations compared to resistant hosts; when host was exposed to high infestations expression of these adhesion molecules was down-regulated in both phenotypes of infestations. Expression of leukocyte adhesion glycoprotein-1 (LFA-1) was higher in skin from susceptible hosts undergoing low or high infestations compared to resistant hosts. Conversely, higher levels of E-selectin, which promotes adhesion of memory T cells, were expressed in skin of resistant animals. This finding may explain the resistant host's ability to mount more rapid and efficient secondary responses that limit hematophagy and infestations. The expression profiles observed for adhesion molecules indicate that there are differences in the kinetics of the inflammatory reactions mounted by resistant and susceptible hosts and the balance between tick and host is affected by the number of tick bites a host receives. We show that the contrasting phenotypes of infestations seen in bovines infested with R. microplus are correlated with differences in the cellular and molecular composition of inflammatory infiltrates elicited by bites with adult ticks. (C) 2009 Published by Elsevier B.V. (AU)