Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

Author(s)

Silva, C. S. ^[1] ; Bovarotti, E. ^[1] ; Rodrigues, M. I. ^[2] ; Hokka, C. O. ^[1] ; Barboza, Marlei ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas, Fac Engn Alimentos, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Bioprocess and Biosystems Engineering; v. 32, n. 5, p. 625-632, AUG 2009.
• Web of Science Citations: 21

Abstract

The purification of clavulanic acid (CA), which is an important beta-lactam antibiotic produced by submerged cultivation of Streptomyces clavuligerus, was studied through the use of phosphate and polyethylene glycol-based aqueous two-phase systems. The parameters' effect on the yield and purification was evaluated through an experimental design and the preliminary results showed that the polyethylene molecular mass and tie-line length and phase volume ratio exerted the strongest effect on the yield and distribution coefficient in the range tested. In addition, the response surface methodology was used to optimize the distribution coefficient, yield, and purification factor. The optimal conditions of yield and purification factor are in the regions where polyethylene has a low molecular mass, pH close to the isoelectric point, and lower top phase volume. A 100% yield and a 1.5-fold purification factor are obtained when extracting CA by maximizing the conditions of an aqueous two-phase system. (AU)

• FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
• Grantee: Carlos Osamu Hokka
• Support Opportunities: Research Projects - Thematic Grants