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(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Beta cell coupling and connexin expression change during the functional maturation of rat pancreatic islets

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Author(s):
Carvalho‚ C.P.F. ; Barbosa‚ H.C.L. ; Britan‚ A. ; Santos-Silva‚ J.C.R. ; Boschero‚ AC ; Meda‚ P. ; Collares-Buzato‚ CB
Total Authors: 7
Document type: Journal article
Source: Diabetologia; v. 53, n. 7, p. 1428-1437, 2010.
Abstract

Cell-cell coupling mediated by gap junctions formed from connexin (CX) contributes to the control of insulin secretion in the endocrine pancreas. We investigated the cellular production and localisation of CX36 and CX43, and gap junction-mediated beta cell coupling in pancreatic islets from rats of different ages, displaying different degrees of maturation of insulin secretion. The presence and distribution of islet connexins were assessed by immunoblotting and immunofluorescence. The expression of connexin genes was evaluated by RT-PCR and quantitative real-time PCR. The ultrastructure of gap junctions and the function of connexin channels were assessed by freeze-fracture electron microscopy and tracer microinjection, respectively. Young and adult beta cells, which respond to glucose, expressed significantly higher levels of Cx36 (also known as Gjd2) than fetal and newborn beta cells, which respond poorly to the sugar. Accordingly, adult beta cells also showed a significantly higher membrane density of gap junctions and greater intercellular exchange of ethidium bromide than newborn beta cells. Cx43 (also known as Gja1) was not expressed by beta cells, but was located in various cell types at the periphery of fetal and newborn islets. These findings show that the pattern of connexins, gap junction membrane density and coupling changes in islets during the functional maturation of beta cells. (AU)

FAPESP's process: 02/13218-0 - Insulin secretion by pancreatic B-cells during pregnancy and the perinatal period of life. Participation of prolactin and insulin signaling cascades in the secretory process and alterations induced by protein malnutrition and disturbances of lipid metabolism
Grantee:Antonio Carlos Boschiero
Support Opportunities: Research Projects - Thematic Grants