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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Assessment of in situ butanol recovery by vacuum during acetone butanol ethanol (ABE) fermentation

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Author(s):
Mariano, Adriano Pinto [1, 2] ; Qureshi, Nasib [3] ; Maciel Filho, Rubens [1] ; Ezeji, Thaddeus Chukwuemeka [2]
Total Authors: 4
Affiliation:
[1] Univ Campinas UNICAMP, Lab Optimizat Design & Adv Control LOPCA, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil
[2] Ohio State Univ, Ohio State Agr Res & Dev Ctr OARDC, Dept Anim Sci, Wooster, OH 44691 - USA
[3] USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 - USA
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY; v. 87, n. 3, p. 334-340, MAR 2012.
Web of Science Citations: 42
Abstract

BACKGROUND: Butanol fermentation is product limiting owing to butanol toxicity to microbial cells. Butanol (boiling point: 118 degrees C) boils at a higher temperature than water (boiling point: 100 degrees C) and application of vacuum technology to integrated acetonebutanolethanol (ABE) fermentation and recovery may have been ignored because of direct comparison of boiling points of water and butanol. This research investigated simultaneous ABE fermentation using Clostridium beijerinckii 8052 and in situ butanol recovery by vacuum. To facilitate ABE mass transfer and recovery at fermentation temperature, batch fermentation was conducted in triplicate at 35 degrees C in a 14 L bioreactor connected in series with a condensation system and vacuum pump. RESULTS: Concentration of ABE in the recovered stream was greater than that in the fermentation broth (from 15.7 g L-1 up to 33 g L-1). Integration of the vacuum with the bioreactor resulted in enhanced ABE productivity by 100% and complete utilization of glucose as opposed to a significant amount of residual glucose in the control batch fermentation. CONCLUSION: This research demonstrated that vacuum fermentation technology can be used for in situ butanol recovery during ABE fermentation and that C. beijerinckii 8052 can tolerate vacuum conditions, with no negative effect on cell growth and ABE production. (C) 2011 Society of Chemical Industry (AU)