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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers

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Author(s):
Guidugli, M. C. [1] ; Ferreira-Ramos, R. [2] ; de Sousa, A. C. B. [3] ; Cidade, F. W. [3] ; Marconi, T. G. [3] ; Mestriner, M. A. [1] ; Groppo, M. [2] ; Alzate-Marin, A. L. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, BR-14049 Ribeirao Preto, SP - Brazil
[3] Univ Estadual Campinas, Dept Genet & Evolucao, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Genetics and Molecular Research; v. 11, n. 1, p. 10-16, 2012.
Web of Science Citations: 7
Abstract

Metrodorea nigra (Rutaceae) is an endemic Brazilian tree of great ecological importance, frequently found in the submontane regions of ombrophilous dense and semideciduous forests. This tree is useful for reforesting degraded areas and the wood can be employed in construction. We developed 12 microsatellite markers from a genomic library enriched for GA/CA repeats, for this species. Polymorphisms were assessed in 40 trees of a highly fragmented population found in Cravinhos, State of Sao Paulo, in southeastern Brazil. Among the 12 loci, 8 were polymorphic and only one had fixed alleles in this population. The number of alleles per locus and expected heterozygosity ranged from 2 to 11 and from 0.190 to 0.889, respectively. These results revealed moderate levels of genetic variation in M. nigra population when compared to other tropical species. Additionally, transferability of the 12 primers was tested in seven other Brazilian Rutaceae tree species (endemics: M. stipularis, Galipea jasminiflora, Esenbeckia leiocarpa and non-endemics: E. febrifuga, E. grandiflora, Balfourodendron riedelianum, Zanthoxylum riedelianum). Transferability ranged among species, but at least 8 loci (similar to 67%) amplified in M. stipularis, demonstrating a high potential for transferring microsatellite markers between species of the same genus in the Rutaceae family. (AU)

FAPESP's process: 08/01559-3 - Mating system, pollen flow and genetic isolation of Aspidosperma polyneuron Mull. Arg. in a fragment situated in São Paulo State
Grantee:Ronai Ferreira Ramos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 06/03170-0 - Phylogenetic and systematic studies in Rutaceae: phylogeny and delimitation of Galipeinae (Galipeae) based on nuclear and plastidial DNA sequences
Grantee:Milton Groppo Júnior
Support type: Research Grants - Young Investigators Grants