| Full text | |
| Author(s): |
Silva, Synara Araujo
[1]
;
Rodrigues, Celia Lima
[1, 2]
;
Campos, Aleia Faustino
[1]
;
Levi, Jose Eduardo
[1, 2]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med, Dept Infect Dis, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Virol Lab, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 54, n. 1, p. 31-35, JAN-FEB 2012. |
| Web of Science Citations: | 2 |
| Abstract | |
The present study aimed at standardizing a real-time quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV RNA. A ``TaqMan{''} assay using primers and probe derived from the 5' NCR region was developed and validated. Two hundred and fifty-three plasma samples from HIV-infected women were tested for GBV-C viremia and antibody against the envelope protein 2. GBV-C RNA was detected in 22.5% of the patients whereas the antibody was identified in 25.3% of the cohort. Detection of viral RNA and of antibodies was mutually exclusive. Viral loads showed a mean of 1,777 arbitrary units/mL, being 1.1 and 13,625 arbitrary units/mL respectively the lowest and highest values measured. We conclude that the real-time quantitative polymerase chain reaction method developed is appropriate for the investigation of GBV-C RNA since it was shown to be highly specific and sensitive, as well as requiring few steps, preventing contamination and providing additional information as to the relative viremia of carriers, a parameter that must be included in studies evaluating the co-factors influencing the clinical outcome of HIV/AIDS. (AU) | |
| FAPESP's process: | 05/01072-9 - GB virus C/HGV and prognosis on HIV infected women |
| Grantee: | José Eduardo Levi |
| Support Opportunities: | Regular Research Grants |