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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigation into in vitro anti-leishmanial combinations of calcium channel blockers and current anti-leishmanial drugs

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Author(s):
Reimao, Juliana Quero [1] ; Tempone, Andre Gustavo [1]
Total Authors: 2
Affiliation:
[1] Adolfo Lutz Inst, Dept Parasitol, BR-01246000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 106, n. 8, p. 1032-1038, DEC 2011.
Web of Science Citations: 8
Abstract

The need for drug combinations to treat visceral leishmaniasis (VL) arose because of resistance to antimonials, the toxicity of current treatments and the length of the course of therapy. Calcium channel blockers (CCBs) have shown anti-leishmanial activity; therefore their use in combination with standard drugs could provide new alternatives for the treatment of VL. In this work, in vitro isobolograms of Leishmania (Leishmania) chagasi using promastigotes or intracellular amastigotes were utilised to identify the interactions between five CCBs and the standard drugs pentamidine, amphotericin B and glucantime. The drug interactions were assessed with a fixed ratio isobologram method and the fractional inhibitory concentrations (FICs), sum of FICs (Sigma FICs) and the overall mean Sigma FIC were calculated for each combination. Graphical isobologram analysis showed that the combination of nimodipine and glucantime was the most promising in amastigotes with an overall mean Sigma FIC value of 0.79. Interactions between CCBs and the anti-leishmanial drugs were classified as indifferent according to the overall mean Sigma FIC and the isobologram graphic analysis. (AU)

FAPESP's process: 08/11434-3 - Therapeutic combinations in visceral leishmaniasis: the antileishmanial potential of calcium channel blockers
Grantee:Juliana Quero Reimão Dalla Zanna
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/09260-7 - Therapeutic combinations for visceral leishmaniasis: the antileishmanial potential of calcium channel blockers and the use of liposomal nanoformulations
Grantee:André Gustavo Tempone Cardoso
Support type: Regular Research Grants