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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Levels of copper in Nile tilapia from Brazil

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Author(s):
Neves, Renato C. F. [1] ; Moraes, Paula M. [2] ; Ferrari, Jeisson E. M. [1] ; Lima, Paula M. [1] ; Santos, Felipe A. [2] ; de Castro, Gustavo Rocha [2] ; Barros, Margarida M. [1] ; Padilha, Pedro M. [2, 3]
Total Authors: 8
Affiliation:
[1] UNESP, Fac Med Vet & Zootecnia, Programa Posgrad Zootecnia, BR-15385000 Botucatu, SP - Brazil
[2] UNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618000 Botucatu, SP - Brazil
[3] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13084971 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE; v. 4, n. 4, p. 238-243, 2011.
Web of Science Citations: 3
Abstract

The purpose of this work was to determine the concentration of copper in samples of farmed Nile tilapia (Oreochromis niloticus L.) fillets purchased in the city of Botucatu (Sao Paulo, Brazil) and in fillet and liver samples of Tilapia fed diets supplemented with different concentrations of Cu from the Laboratory of Aquatic Organism Nutrition/FMVZ-UNESP (Botucatu, Brazil). The fillet samples were prepared by lyophilisation and cryogenic grinding into particles smaller than 60 mm, and copper was extracted ultrasonically using 0.10 mol l(-1) HCl as extraction solution. Copper determination was performed by graphite furnace atomic absorption spectrometry (GFAAS) with optimised temperatures of drying, pyrolysis, atomisation and cleaning. Palladium nitrate was injected into the samples as a chemical modifier and tungsten as a permanent modifier. Copper concentrations of 0.70-1.60mg kg(-1) were found, which are in line with Brazilian regulations. The accuracy and precision of the copper concentrations determined in this study were evaluated using certified standard Lake Michigan fish tissue (NIST SRM 1947) (AU)

FAPESP's process: 07/59778-0 - Development of analytical methods for evaluation of Nile tilapia (Oreochromis niloticus) metalloproteins
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants