| Full text | |
| Author(s): |
Maurer-Morelli, Claudia V.
;
de Vasconcellos, Jaira F.
;
Reis-Pinto, Fernanda C.
;
Rocha, Cristiane de S.
;
Domingues, Romenia R.
;
Yasuda, Clarissa L.
[1]
;
Tedeschi, Helder
[1]
;
De Oliveira, Evandro
[1]
;
Cendes, Fernando
[1]
;
Lopes-Cendes, Iscia
[2]
Total Authors: 10
|
| Affiliation: | [1] Univ Campinas UNICAMP, Dept Neurol, BR-13083887 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Med Sci, Dept Med Genet, BR-13083887 Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF NEUROSCIENCE METHODS; v. 208, n. 1, p. 44-47, JUN 30 2012. |
| Web of Science Citations: | 8 |
| Abstract | |
The reliability of gene expression studies by mRNA quantification is highly dependent upon several experimental procedures, including the choice of reference genes used for data normalization. In order to contribute to gene expression studies in mesial temporal lobe epilepsy (MTLE) we used microarray data, followed by real time quantitative PCR validation of selected housekeeping genes, to determine the most appropriate reference genes to be used in human hippocampal tissue gene expression studies. Our results unequivocally showed a significant impact of the reference gene chosen for normalization on the overall results of expression studies, clearly demonstrating the importance of adequate validation using stable reference genes. In addition, we found that HPRT, NSE, SDHA and SYP are suitable genes to be used as reference for normalization in expression studies of hippocampal tissue obtained from patients with MTLE. (C) 2012 Published by Elsevier B.V (AU) | |
| FAPESP's process: | 05/56578-4 - Multimodal neuroimaging center for studies in epilepsy |
| Grantee: | Fernando Cendes |
| Support Opportunities: | Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants |
| FAPESP's process: | 08/54789-6 - Análise da expressão gênica na epilepsia de lobo temporal mesial através da técnica de chip de DNA em espécimes cirúrgicos hipocampais de pacientes refratários à medicação e em tecido cerebral de modelos |
| Grantee: | Cláudia Vianna Maurer Morelli |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/17440-5 - Análise de expressão gênica em larga escala na epilepsia de lobo temporal mesial |
| Grantee: | Jaíra Ferreira de Vasconcellos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |