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(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased Percentages of T Helper Cells Producing IL-17 and Monocytes Expressing Markers of Alternative Activation in Patients with Sepsis

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Author(s):
Colo Brunialti, Milena Karina [1] ; Santos, Michelle Carolina [1] ; Rigato, Otelo [1, 2] ; Machado, Flavia Ribeiro [3] ; Silva, Eliezer [4] ; Salomao, Reinaldo [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Div Infect Dis, Dept Med, Escola Paulista Med, Hosp Sao Paulo, Sao Paulo - Brazil
[2] Hosp Sirio Libanes, Crit Care Unit, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Div Anesthesiol, Dept Surg, Escola Paulista Med, Hosp Sao Paulo, Sao Paulo - Brazil
[4] Hosp Albert Einstein, Crit Care Unit, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 7, n. 5, p. e37393, 2012.
Web of Science Citations: 11
Abstract

Background: A shift from Th1 to Th2 as well as an increase in Treg CD4+T cell subsets has been reported in septic patients (SP). Furthermore, these patients display modulation of monocyte function, with reduced production of pro-inflammatory cytokines upon LPS stimulus, which resembles the phenotype of alternatively activated macrophages. In this study, we evaluated the percentages of T cells differentiated into Th1, Th17 and Treg subsets, as well as the percentage of monocytes expressing markers of alternatively activated monocytes/macrophages (AAM) in SP. Methodology/Principal Findings: Peripheral blood mononuclear cells (PBMC) were obtained from 32 healthy volunteers (HV) and from SP at admission (D0, n=67) and after 7 days of therapy (D7, n=33). Th1 and Th17 (CD3+CD8-) lymphocytes were identified by the intracellular detection of IFN-gamma and IL-17, respectively, spontaneously and after PMA/Io stimulation, and Treg cells were identified by Foxp3+CD127-expression. Monocytes were evaluated for CD206 and CD163 expression. Absolute numbers of CD4+T lymphocytes were measured in whole blood samples by flow cytometry. The Mann-Whitney or Wilcoxon test was applied, as appropriate. The percentage of Th1 cells was lower in SP than in HV at admission after PMA/Io stimulation, whereas the percentage of Th17 cells was higher. In patients' follow-up samples, a higher percentage of Th1 cells and a lower percentage of Th17 cells were observed on D7 compared with the D0 samples. Treg cells remained unchanged. Septic patients showed a markedly increased proportion of monocytes expressing CD163 and CD206. Conclusions/Significance: Upon in vitro stimulus, the percentage of T helper lymphocytes producing IL-17 was higher in SP than in HV at admission, and the percentage producing IFN-gamma was lower, a pattern that was reversed during follow-up. The increased expression of CD163 and CD206 indicates that monocytes may acquire the AAM phenotype during sepsis. (AU)

FAPESP's process: 06/58744-1 - Sepsis: integrating basic research and clinical investigation
Grantee:Reinaldo Salomão
Support Opportunities: Research Projects - Thematic Grants