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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HEV infection in swine from Eastern Brazilian Amazon: Evidence of co-infection by different subtypes

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Souza de Souza, Alex Junior [1, 2] ; Gomes-Gouvea, Michele Soares [3, 4] ; Pereira Soares, Manoel do Carmo [1] ; Rebello Pinho, Joao Renato [3, 4] ; Malheiros, Andreza Pinheiro [1] ; Carneiro, Liliane Almeida [1] ; Lopes dos Santos, Debora Regina [5] ; Assuncao Pereira, Washington Luiz [6]
Total Authors: 8
[1] Inst Evandro Chagas, Secao Hepatol, BR-66093020 Belem, PA - Brazil
[2] Fac Integradas Tapajos, Ctr Ciencias Biol & Saude, BR-68010200 Santarem, PA - Brazil
[3] Univ Sao Paulo, Sch Med, Sao Paulo Inst Trop Med, Lab Gastroenterol & Hepatol, BR-05508 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Gastroenterol, BR-05508 Sao Paulo - Brazil
[5] Univ Fed Rural Rio de Janeiro, Inst Vet, BR-23890000 Rio De Janeiro, RJ - Brazil
[6] Univ Fed Rural Amazonia, Inst Saude & Prod Anim, BR-66077901 Belem, PA - Brazil
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 24

Hepatitis E virus (HEV) is a fecal-orally transmitted member of the genus Hepevirus that causes acute hepatitis in humans and is widely distributed throughout the world. Pigs have been reported as the main source of genotypes 3 and 4 infection to humans in non-endemic areas. To investigate HEV infection in pigs from different regions of Para state (Eastern Brazilian Amazon), we performed serological and molecular analyses of serum, fecal and liver samples from 151 adult pigs slaughtered between April and October 2010 in slaughterhouses in the metropolitan region of Belem, Para. Among the animals tested, 8.6% (13/151) were positive for anti-HEV IgG but not for anti-HEV IgM. HEV RNA was detected in 4.8% (22/453) of the samples analyzed and 9.9% (15/151) of the animals had at least one positive sample. Phylogenetic analysis showed that all sequences belonged to genotype 3 that were related to human isolates from other non-endemic regions, suggesting that the isolates had zoonotic potential. Subtypes 3c and 3f were simultaneously detected in some pigs, suggesting co-infection by more than one strain and/or the presence of a recombinant virus. These results constitute the first molecular and serologic evidence of swine HEV circulation in the Eastern Brazilian Amazon. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 09/53946-3 - Acquisition of a genetic analyzer for automated DNA sequencing
Grantee:João Renato Rebello Pinho
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 10/50081-9 - Prevalence of primary resistance to antiviral therapy against hepatitis B in patients with chronic hepatitis B virus infection and not submitted to treatment
Grantee:Michele Soares Gomes Gouvêa
Support Opportunities: Scholarships in Brazil - Doctorate