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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MICROBIOLOGICAL EVALUATION OF DIFFERENT STRATEGIES FOR MANAGEMENT OF SNAKES IN CAPTIVITY

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Author(s):
Campagner, M. V. [1, 2] ; Bosco, S. M. G. [3] ; Bagagli, E. [3] ; Cunha, M. L. R. S. [3] ; Jeronimo, B. C. [2] ; Saad, E. [1, 2] ; Biscola, N. P. [1, 2] ; Ferreira, Jr., R. S. [1, 2] ; Barraviera, B. [1, 2]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Trop Dis, Jaboticabal, SP - Brazil
[2] Sao Paulo State Univ UNESP Univ Estadual Paulista, Ctr Study Venoms & Venomous Anim CEVAP, Jaboticabal, SP - Brazil
[3] Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Biosci Inst, Dept Microbiol & Immunol, Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 75, n. 16-17, SI, p. 1070-1080, 2012.
Web of Science Citations: 3
Abstract

Keeping snakes in captivity to produce venom for scientific research and production of inputs is now a worldwide practice. Maintaining snakes in captivity involves capture, infrastructure investments, management techniques, and appropriate qualified personnel. Further, the success of the project requires knowledge of habitat, nutrition, and reproduction, and control of opportunistic infections. This study evaluated the management of snakes in three types of captivity (quarantine, intensive, and semiextensive) and diagnosed bacterial and fungal contaminants. A bacteriological profile was obtained by swabbing the oral and cloacal cavities, scales, and venoms of healthy adult snakes from Bothrops jararaca (Bj) and Crotalus durissus terrificus (Cdt). There was predominance of Enterobacteriaceae, especially non-fermenting Gram-negative bacilli excluding Pseudomonas spp and Gram- positive bacteria. Statistically, intensive captivity resulted in the highest number of bacterial isolates, followed by recent capture (quarantine) and by semiextensive captivity. No statistical difference was found between Bj and Cdt bacterial frequency. In vitro bacterial susceptibility testing found the highest resistance against the semisynthetic penicillins (amoxicillin and ampicillin) and highest sensitivity to amicacin and tobramycin aminoglycosides. To evaluate mycological profile of snakes from intensive captivity, samples were obtained from two healthy Bj and one B. moojeni, one B. pauloensis, and one Cdt showing whitish lesions on the scales suggestive of ringworm. Using conventional methods and DNA-based molecular procedures, five samples of Trichosporon asahii were identified. Despite the traditional role of intense captivity in ophidian venom production, semiextensive captivity was more effective in the present study by virtue of presenting superior control of bacterial and fungal transmission, easier management, lowest cost, and decreased rate of mortality; therefore, it should be considered as a good alternative for tropical countries. (AU)

FAPESP's process: 09/06280-0 - Sexual, ontogenetic and enviromental variation in the Venom of Crotalus durissus terrificus and Bothrops jararaca on Botucatu region - São Paulo State - Brazil: pharmacological, biochemical and Enzymatical characterization
Grantee:Rui Seabra Ferreira Junior
Support type: Regular Research Grants
FAPESP's process: 09/53846-9 - Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant
Grantee:Benedito Barraviera
Support type: Multi-user Equipment Program