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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oxidative stress modulates DNA methylation during melanocyte anchorage blockade associated with malignant transformation

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Campos, Ana C. E. ; Molognoni, Fernanda ; Melo, Fabiana H. M. ; Galdieri, Luciano C. ; Carneiro, Célia R. W. ; D'Almeida, Vânia ; Correa, Mariangela ; Jasiulionis, Miriam G.
Total Authors: 8
Document type: Journal article
Source: Neoplasia; v. 9, n. 12, p. 1111-1121, Dec. 2007.
Field of knowledge: Biological Sciences - Microbiology

Both oxidative/nitrosative stress and alterations in DNA methylation are observed during carcinogenesis of different tumor types, but no clear correlation between these events has been demonstrated until now. Melanoma cell lines were previously established after submitting the nontumorigenic melanocyte lineage, melan-a, to cycles of anchorage blockade. In this work, increased intracellular oxidative species and nitric oxide levels, as well as alterations in the DNA methylation, were observed after melan-a detachment, which were also associated with a decrease in intracellular homocysteine (Hcy), an element in the methionine (universal methyl donor) cycle. This alteration was accompanied by increase in glutathione (GSH) levels and methylated DNA content. Furthermore, a significant increase in dnmt1 and 3b expression was identified along melan-a anchorage blockade. lG-Nitro-l-arginine methyl esther (l-NAME), known as a nitric oxide synthase (NOS) inhibitor, and N-acetyl-l-cysteine (NAC) prevented the increase in global DNA methylation, as well as the increase in dnmt1 and 3b expression, observed during melan-a detachment. Interestingly, both l-NAME and NAC did not inhibit nitric oxide (NO) production in these cells, but abrogated superoxide anion production during anchorage blockade. In conclusion, oxidative stress observed during melanocyte anchorage blockade seems to modulate DNA methylation levels and may directly contribute to the acquisition of an anoikis-resistant phenotype through an epigenetic mechanism. (AU)

FAPESP's process: 06/61293-1 - DNA methylation contribution to carcinogenesis
Grantee:Miriam Galvonas Jasiulionis
Support type: Research Grants - Young Investigators Grants