| Full text | |
| Author(s): |
Matos, Gabriela
[1]
;
Ribeiro, Daniel A.
[2]
;
Alvarenga, Tathiana A.
[1]
;
Hirotsu, Camila
[1]
;
Scorza, Fulvio A.
[3]
;
Le Sueur-Maluf, Luciana
[2]
;
Noguti, Juliana
[4]
;
Cavalheiro, Esper A.
[3]
;
Tufik, Sergio
[1]
;
Andersen, Monica L.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04024002 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-04024002 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Neurol Expt, BR-04024002 Sao Paulo - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Patol, BR-04024002 Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Neuroscience Letters; v. 515, n. 2, p. 137-140, MAY 2 2012. |
| Web of Science Citations: | 8 |
| Abstract | |
The interaction between sleep deprivation and epilepsy has been well described in electrophysiological studies, but the mechanisms underlying this association remain unclear. The present study evaluated the effects of sleep deprivation on locomotor activity and genetic damage in the brains of rats treated with saline or pilocarpine-induced status epilepticus (SE). After 50 days of pilocarpine or saline treatment, both groups were assigned randomly to total sleep deprivation (TSD) for 6 h, paradoxical sleep deprivation (PSD) for 24 h, or be kept in their home cages. Locomotor activity was assessed with the open field test followed by resection of brain for quantification of genetic damage by the single cell gel electrophoresis (comet) assay. Status epilepticus induced significant hyperactivity in the open field test and caused genetic damage in the brain. Sleep deprivation procedures (TSD and PSD) did not affect locomotor activity in epileptic or healthy rats, but resulted in significant DNA damage in brain cells. Although PSD had this effect in both vehicle and epileptic groups. TSD caused DNA damage only in epileptic rats. In conclusion, our results revealed that, despite a hack of behavioral effects of sleep deprivation. TSD and PSD induced genetic damage in rats submitted to pilocarpine-induced SE. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 09/01030-5 - Effect of sleep deprivation and sleep restriction on the reproductive function of male rats |
| Grantee: | Tathiana Aparecida Fernandes de Alvarenga |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 98/14303-3 - Center for Sleep Studies |
| Grantee: | Sergio Tufik |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 10/15110-8 - Sleep and sudden unexpected death in epilepsy: possible molecular basis? |
| Grantee: | Gabriela de Matos Barbosa Pimenta |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/50129-1 - Insuficiência renal crônica, restrição de sono e sildenafil: consequências renais, cardíacas e sexuais em um modelo animal |
| Grantee: | Camila Hirotsu |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |