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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

N-glycome profiling of Bothrops jararaca newborn and adult venoms

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Author(s):
Zelanis, Andre [1, 2] ; Serrano, Solange M. T. [1, 2] ; Reinhold, Vernon N. [3]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil
[2] Inst Butantan, Lab Especial Toxinol Aplicada CAT Cepid, BR-05503000 Sao Paulo - Brazil
[3] Univ New Hampshire, Glyc Ctr, Dept Chem, Durham, NH 03824 - USA
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 75, n. 3, p. 774-782, JAN 4 2012.
Web of Science Citations: 22
Abstract

Glycosylation is an important post-translational modification of snake venom proteins and contributes to venom proteome complexity. Many snake venom components are known to be glycosylated, however, very little is known about the carbohydrate structures present in venom glycoproteins. Previous studies showed that the ontogenetic shift in diet, from ectothermic prey in early life to endothermic prey in adulthood, and shift in animal size are associated with changes in the venom proteome of the snake Bothrops jararaca. In this study we explored the composition of the N-glycome released from newborn and adult B. jararaca venom proteins. We used an ion trap mass spectrometer (IT-MS) to disassemble glycan structures based on the use of several pathways of MS (MSn) and demonstrate the presence of some structural isomers in both newborn and adult venom B. jararaca N-glycans. The main N-glycans identified in both venoms are of the hybrid/complex type however some mannose-rich type structures were also detected. The N-glycan composition of newborn and adult venoms did not vary indicating that differences in the utilization of the N-glycosylation motif could be the explanation for the differences in the glycosylation levels indicated by the differential electrophoretic profiles previously reported for B. jararaca newborn and adult venoms. (C) 2011 Elsevier B.V. All rights reserved. (AU)