| Full text | |
| Author(s): |
Borin Scutti, Jorge A.
[1]
;
Matsuo, Alisson Leonardo
[1]
;
Pereira, Felipe Valenca
[1]
;
Massaoka, Mariana Hiromi
[1]
;
Figueiredo, Carlos Rogerio
[1]
;
Moreira, Dayson Friaca
[2]
;
Belizario, Jose Ernesto
[2]
;
Travassos, Luiz R.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Unidade Oncol Expt, BR-04023062 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Pharmacol, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | TRANSLATIONAL ONCOLOGY; v. 4, n. 2, p. 101-109, APR 2011. |
| Web of Science Citations: | 17 |
| Abstract | |
Melanoma is the most aggressive form of skin cancer, and its incidence has increased dramatically over the years. The murine B16F10 melanoma in syngeneic C57Bl/6 mice has been used as a highly aggressive model to investigate tumor development. Presently, we demonstrate in the B16F10-Nex2 subclone that silencing of SOCS-1, a negative regulator of Jak/Stat pathway, leads to reversal of the tumorigenic phenotype and inhibition of melanoma cell metastasis. SOCS-1 silencing with short hairpin RNA affected tumor growth and cell cycle regulation with arrest at the S phase with large-sized nuclei, reduced cell motility, and decreased melanoma cell invasion through Matrigel. A clonogenic assay showed that SOCS-1 acted as a modulator of resistance to anoikis. In addition, down-regulation of SOCS-1 decreased the expression of epidermal growth factor receptor ( mainly the phosphorylated-R), Ins-R alpha, and fibroblast growth factor receptor. In vivo, silencing of SOCS-1 inhibited subcutaneous tumor growth and metastatic development in the lungs. Because SOCS-1 is expressed in most melanoma cell lines and bears a relation with tumor invasion, thickness, and stage of disease, the present results on the effects of SOCS-1 silencing in melanoma suggest that this regulating protein can be a target of cancer therapy. (AU) | |
| FAPESP's process: | 06/50634-2 - Peptides and peptidases: biological activities in infectious diseases and cancer |
| Grantee: | Luiz Rodolpho Raja Gabaglia Travassos |
| Support Opportunities: | Research Projects - Thematic Grants |