Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenotypic and molecular characterization of recent and archived Erysipelothrix spp. isolated from Brazilian swine

Full text
Author(s):
Coutinho, Tania A. [1] ; Imada, Yumiko [2] ; Barcellos, David E. S. N. [3] ; Oliveira, Sergio J. [4] ; Moreno, Andrea M. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, FMVZ, Lab Sanidade Suina & Virol, BR-05508270 Sao Paulo - Brazil
[2] Natl Inst Anim Hlth, Dept Biol Prod, Tsukuba, Ibaraki 305 - Japan
[3] Univ Fed Rio Grande do Sul, Fac Vet FAVET, Setor Suinos, Porto Alegre, RS - Brazil
[4] Univ Luterana Brasil ULBRA, Fac Vet, Lab Microbiol Clin & Alimentos, Canoas - Brazil
Total Affiliations: 4
Document type: Journal article
Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 69, n. 2, p. 123-129, FEB 2011.
Web of Science Citations: 6
Abstract

One hundred fifty-one Erysipelothrix spp. isolates from Brazilian swine were characterized by serotyping, determination of antimicrobial susceptibility, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE). Among all isolates, 139 were classified in 18 different serotypes and serotype 2b was the most frequent. The susceptibility profiles of the isolates were very similar among each other, which did not permit subtyping Erysipelothrix spp. isolates by the antimicrobial susceptibility testing. Despite the fact that AFLP and PFGE provided the same discriminatory index (0.98), PFGE was more discriminatory than AFLP, given the types of groups it generates. Regardless the technique employed (AFLP or PFGE), no discrimination between recent and historical isolates was established, neither a fixed epidemiologic pattern for their grouping was observed. Nevertheless, AFLP could be an interesting alternative for discriminating the Erysipelothrix species, while PFGE could be an indication for discerning this bacterium according to the serotypes. (C) 2011 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 06/55502-7 - Characterization of Erysipelothrix spp. strains isolated from swine in last 30 years
Grantee:Tania Alen Coutinho
Support type: Scholarships in Brazil - Doctorate