Monolayer formation of human osteoblastic cells on vertically aligned multiwalled carbon nanotube scaffolds

Author(s)

Lobo, Anderson O. ^{[1, 2]} ; Antunes, Erica F. ^{[1, 2]} ; Palma, Mariana B. S. ^[2] ; Pacheco-Soares, Cristina ^[3] ; Trava-Airoldi, Vladimir J. ^{[1, 2]} ; Corat, Evaldo J. ^{[1, 2]}

Affiliation

^[1] Inst Tecnol Aeronaut CTA, BR-12228900 Sao Jose Dos Campos, SP - Brazil

^[2] Inst Nacl Pesquisas Espaciais, Lab Associado Sensores & Mat, BR-12245970 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Lab Dinam Compartimentos Celulares, BR-12227010 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Cell Biology International; v. 34, n. 4, p. 393-398, APR 2010.
• Web of Science Citations: 8

Abstract

Mono layer formation of SaOS-2 (human osteoblast-like cells) was observed on VACNT (vertically aligned multiwalled carbon nanotubes) scaffolds without purification or functionalization. The VACNT were produced by a microwave plasma chemical vapour deposition on titanium surfaces with nickel or iron as catalyst. Cell viability and morphology studies were evaluated by LDH (lactate dehydrogenase) release assay and SEM (scanning electron microscopy), respectively. The non-toxicity and the flat spreading with monolayer formation of the SaOs-2 on VACNT scaffolds surface indicate that they can be used for biomedical applications. (AU)

• FAPESP's process: 07/00013-4 - New materials, studies and applications of CVD diamond, diamond-like-carbon (DLC) and nanostructured carbon obtained by chemical vapor deposition
• Grantee: Evaldo Jose Corat
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 08/11642-5 - Development of structures based on carbon nanotubes, bioresorbable polymers and carbon fibers for studies with regenerative cells and hematopoietic stem cells
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Scholarships in Brazil - Doctorate