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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial cellulose-collagen nanocomposite for bone tissue engineering

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Saska, Sybele [1] ; Teixeira, Lucas Novaes [2] ; de Oliveira, Paulo Tambasco [2] ; Minarelli Gaspar, Ana Maria [3] ; Lima Ribeiro, Sidney Jose [1] ; Messaddeq, Younes [1] ; Marchetto, Reinaldo [1]
Total Authors: 7
[1] Univ Estadual Paulista Unesp, Inst Chem, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Dent Ribeirao Preto, Cell Culture Lab, BR-14040904 Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Sch Dent, Dept Morphol, BR-14801903 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Materials Chemistry; v. 22, n. 41, p. 22102-22112, NOV 7 2012.
Web of Science Citations: 60

A nanocomposite based on bacterial cellulose (BC) and type I collagen (COL) was evaluated for in vitro bone regeneration. BC membranes were modified by glycine esterification followed by cross-linking of type I collagen employing 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Collagen incorporation was studied by spectroscopy analysis. X-Ray diffraction showed changes in the BC crystallinity after collagen incorporation. The elastic modulus and tensile strength for BC-COL decreased, while the strain at failure showed a slight increase, even after sterilization, as compared to pristine BC. Swelling tests and contact angle measurements were also performed. Cell culture experiments performed with osteogenic cells were obtained by enzymatic digestion of newborn rat calvarium revealed similar features of cell morphology for cultures grown on both membranes. Cell viability/proliferation was not different between BC and BC-COL membranes at day 10 and 14. The high total protein content and ALP activity at day 17 in cells cultured on BC-COL indicate that this composite allowed the development of the osteoblastic phenotype in vitro. Thus, BC-COL should be considered as alternative biomaterial for bone tissue engineering. (AU)

FAPESP's process: 09/09960-1 - Nanocomposites based on bacterial cellulose for application in the bone tissue regeneration
Grantee:Reinaldo Marchetto
Support type: Regular Research Grants