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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crystallization and preliminary diffraction analysis of the catalytic domain of major extracellular endoglucanase from Xanthomonas campestris pv. campestris

Full text
Author(s):
Rosseto, Flavio R. [1] ; Puhl, Ana C. [1] ; Andrade, Maxuel O. [2] ; Polikarpov, Igor [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 69, n. 2, p. 137-140, FEB 2013.
Web of Science Citations: 2
Abstract

Cellulases, such as endoglucanases, exoglucanases and beta-glucosidases, are important enzymes used in the process of enzymatic hydrolysis of plant biomass. The bacteria Xanthomonas campestris pv. campestris expresses a large number of hydrolases and the major endoglucanase (XccEG), a member of glycoside hydrolase family 5 (GH5), is the most strongly secreted extracellularly. In this work, the native XccEG was purified from the extracellular extract and crystallization assays were performed on its catalytic domain. A complete data set was collected on an in-house X-ray source. The crystal diffracted to 2.7 angstrom resolution and belonged to space group C2, with unit-cell parameters a = 174.66, b = 141.53, c = 108.00 angstrom, beta = 110.49 degrees. The Matthews coefficient suggests a solvent content of 70.1% and the presence of four protein subunits in the asymmetric unit. (AU)

FAPESP's process: 09/05349-6 - Expression and purification of cellobiohydrolase I from the filamentous fungus Trichoderma harzianum
Grantee:Bruno Luan Soares Paula de Mello
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 07/08706-9 - Molecular and structural studies of Cellulitic complex enzymes of fungi Trichoderma harzianum with potencial of enzymatic transformation of biomass
Grantee:Viviane Isabel Serpa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis
Grantee:Igor Polikarpov
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery
Grantee:Igor Polikarpov
Support type: Regular Research Grants