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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors

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Author(s):
Rodrigues Vilardi, Bruna Maria [1] ; Bravo-Calderon, Diego Mauricio [1] ; Bernabe, Daniel Galera [2, 3] ; Penha Oliveira, Sandra Helena [4] ; Oliveira, Denise Tostes [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Area Pathol, BR-17012901 Sao Paulo - Brazil
[2] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Oral Oncol Ctr, Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Pathol & Clin Propedeut, Sao Paulo - Brazil
[4] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Basic Sci, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TUMOR BIOLOGY; v. 34, n. 1, p. 139-143, FEB 2013.
Web of Science Citations: 5
Abstract

The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 mu M) and 1 mu M of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. (AU)

FAPESP's process: 10/06333-3 - Modulation of VEGF-C expression by stress related mediator in oral squamous cell carcinoma cell lines
Grantee:Denise Tostes Oliveira
Support Opportunities: Regular Research Grants