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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer

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Jorge, Yvana Cristina [1] ; Mataruco, Mayra Mioto [1] ; Araujo, Leandro Pires [1] ; Teixeira Rossi, Ana Flavia [1] ; de Oliveira, Juliana Garcia [1] ; Valsechi, Marina Curado [1] ; Caetano, Alaor [2] ; Miyazaki, Kenji [3] ; de Jesus Fazzio, Celia Sebastiana [4, 5] ; Thome, Jorge Alberto [6] ; Rahal, Paula [1] ; Oliani, Sonia Maria [1] ; Silva, Ana Elizabete [1]
Total Authors: 13
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Rio Preto Endoscopy Ctr, Sao Jose Do Rio Preto, SP - Brazil
[3] Hosp Base, Endoscopy Serv, Sao Jose Do Rio Preto, SP - Brazil
[4] Hosp Base, Dept Legal Med, Sao Jose Do Rio Preto, SP - Brazil
[5] Hosp Base, Pathol Serv, Sao Jose Do Rio Preto, SP - Brazil
[6] Inst Anat Pathol & Cytopathol, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Mediators of Inflammation; 2013.
Web of Science Citations: 21
Abstract

Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26 +/- 2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38 +/- 4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44 +/- 3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43 +/- 3.13; P < 0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis. (AU)

FAPESP's process: 12/15036-8 - Evaluation of gene and protein expression in inflammatory processes and neoplasms of the digestive tract: stomach and colorectal
Grantee:Ana Elizabete Silva
Support type: Regular Research Grants