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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photodynamic Inactivation of Planktonic Cultures and Biofilms of Candida albicans Mediated by Aluminum-Chloride-Phthalocyanine Entrapped in Nanoemulsions

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Dias Ribeiro, Ana Paula [1] ; Andrade, Mariana Carvalho [1] ; da Silva, Julhiany de Fatima [2] ; Jorge, Janaina Habib [1] ; Primo, Fernando Lucas [3] ; Tedesco, Antonio Claudio [3] ; Pavarina, Ana Claudia [1]
Total Authors: 7
[1] UNESP Univ Estadual Paulista, Dept Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Jaboticabal, SP - Brazil
[2] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil
[3] FFCLRP Sao Paulo Univ, Ctr Nanotechnol & Tissue Engineers, Photobiol & Photomed Res Grp, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Photochemistry and Photobiology; v. 89, n. 1, p. 111-119, JAN-FEB 2013.
Web of Science Citations: 22

New drug delivery systems, such as nanoemulsions (NE), have been developed to allow the use of hydrophobic drugs on the antimicrobial photodynamic therapy. This study evaluated the photodynamic potential of aluminum-chloride-phthalocyanine (ClAlPc) entrapped in cationic and anionic NE to inactivate Candida albicans planktonic cultures and biofilm compared with free ClAlPc. Fungal suspensions were treated with different delivery systems containing ClAlPc and light emitting diode. For planktonic suspensions, colonies were counted and cell metabolism was evaluated by XTT assay. Flow cytometry evaluated cell membrane damage. For biofilms, the metabolic activity was evaluated by XTT and ClAlPc distribution through biofilms was analyzed by confocal laser scanning microscopy (CLSM). Fungal viability was dependent on the delivery system, superficial charge and light dose. Free ClAlPc caused photokilling of the yeast when combined with 100 J cm-2. Cationic NE-ClAlPc reduced significantly both colony counts and cell metabolism (P < 0.05). In addition, cationic NE-ClAlPc and free ClAlPc caused significant damage to the cell membrane (P < 0.05). For the biofilms, cationic NE-ClAlPc reduced cell metabolism by 70%. Anionic NE-ClAlPc did not present antifungal activity. CLSM showed different accumulation on biofilms between the delivery systems. Although NE system showed a lower activity for planktonic culture, cationic NE-ClAlPc showed better results for Candida biofilms. (AU)

FAPESP's process: 08/03994-9 - Study of the effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the intracelular damage caused in yeast cells: in vitro and in vivo evaluation of two photosensitizers
Grantee:Ana Cláudia Pavarina
Support Opportunities: Regular Research Grants
FAPESP's process: 10/05425-1 - Evaluation of the effectiveness of antimicrobial photodynamic therapy in the inactivation of Candida species and cellular damage caused to skin cells: in vitro and in vivo evaluation of the aluminum-chloro-phtalocyanine and other derivatives
Grantee:Ana Cláudia Pavarina
Support Opportunities: Regular Research Grants